Acute, rapid, and chronic tolerance during ontogeny: Observations when equating ethanol perturbation across age
Acute, rapid, and chronic tolerance during ontogeny: Observations when equating ethanol perturbation across age
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DOI：
10.1111/j.1530-0277.2001.tb02351.x
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发表时间：
2001-09-01
影响因子：
3.2
通讯作者：
Spear, LP
中科院分区：
文献类型：
--
作者：
Silveri, MM;Spear, LP
背景：在发育过程中，对乙醇（EtOH）的运动损伤和催眠作用的敏感性显著增加。然而，关于EtOH耐受性的个体发生和不同类型耐受性之间的个体发生关系知之甚少。因此，我们比较了在游泳任务中对EtOH诱导的运动损伤和低温的急性、快速和慢性耐受性的个体发育。断奶前、青春期和成年雌性和雄性Sprague-Dawley大鼠给予慢性生理盐水（对照组），在试验日EtOH前每日5次EtOH暴露（慢性组）、在测试日之前暴露一次EtOH（快速组）或仅在测试日暴露EtOH（急性组）。在注射后15、60或105 min对急性组中的单独动物组进行检测，以通过计算每个注射后间隔时损伤相对于脑酒精水平的线性回归斜率来估计急性耐受性发展。初始EtOH扰动的游泳性能等同于不同年龄的EtOH dose.Results：急性耐受性是明显的电动机损害的影响，EtOH在所有年龄。当损伤指数相对于大脑酒精水平，快速和慢性耐受性的运动损伤的影响，乙醇的潜伏期，以达到开始被视为跨年龄，虽然这种耐受性往往是更明显的成年人。有些不同的个体发育模式的耐受性发展观察EtOH诱导的低温，一个依赖的措施，其中EtOH扰动不等于across age.Conclusions：EtOH的初始扰动的程度似乎是一个重要的预测个体发育过程中的耐受性表达。也就是说，当EtOR诱导的运动损伤在年龄上等同而不是在EtOH给药剂量上等同时，耐受性发展的个体发育特征显著不同。在探索EtOH耐受性的个体发育表达时，还应考虑目标反应措施和环境压力的作用。
Background: Sensitivity to the motor-impairing and hypnotic effects of ethanol (EtOH) increases notably during development. Less is known, however, about the ontogeny of EtOH tolerance and the ontogenetic relationship among different types of tolerance. Consequently, we compared the ontogenetic development of acute, rapid, and chronic tolerance to EtOH-induced motor impairment and hypothermia in a swim task.Methods: Preweanling, adolescent, and adult female and male Sprague-Dawley rats were given chronic saline (control group), five daily EtOH exposures before EtOH on test day (chronic group), one EtOH exposure before test day (rapid group), or EtOH exposure only on test day (acute groups). Separate groups of animals in the acute groups were tested at 15, 60, or 105 min after injection to estimate acute tolerance development via calculating slopes of the linear regression of impairment relative to brain alcohol levels at each postinjection interval. Initial EtOH perturbation of swim performance was equated across age by varying EtOH dose.Results: Acute tolerance was evident to the motor-impairing effects of EtOH at all ages. When impairment was indexed relative to brain alcohol levels, rapid and chronic tolerance to the motor-impairing effects of EtOH on latency to reach the start was seen across age, although this tolerance tended to be more pronounced in adults. Somewhat different ontogenetic patterns of tolerance development were observed with EtOH-induced hypothermia, a dependent measure for which EtOH perturbation was not equated across age.Conclusions: The degree of initial perturbation by EtOH seems to be an important predictor of tolerance expression during ontogeny. That is, ontogenetic profiles of tolerance development differ significantly when EtOR-induced motor impairment is equated across age rather than dose of EtOH administered. The role of target response measures and context stress should also be considered when exploring ontogenetic expression of EtOH tolerance.