Differential Effect of Solar Light in Increasing the Toxicity of Silver and Titanium Dioxide Nanoparticles to a Fish Cell Line and Zebrafish Embryos
Differential Effect of Solar Light in Increasing the Toxicity of Silver and Titanium Dioxide Nanoparticles to a Fish Cell Line and Zebrafish Embryos
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DOI：
10.1021/es405768n
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发表时间：
2014-06-03
影响因子：
11.4
通讯作者：
Chan, Woon Khiong
中科院分区：
文献类型：
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作者：
George, Saji;Gardner, Hannah;Chan, Woon Khiong
消费品中越来越多地使用银和二氧化钛纳米颗粒（NPs）及其不可避免地渗透到环境中，促使我们研究其在黑暗和模拟太阳光（SSL）暴露条件下对鱼细胞系（BF-2）和斑马鱼胚胎的潜在毒性。使用高通量筛选（HTS）平台，我们发现，氧化应激依赖的细胞毒性和胚胎毒性的纳米颗粒暴露于SSL后显着增加。SSL暴露下TiO 2 NPs的毒性可以通过产生羟基自由基来解释，SSL暴露下Ag NPs的毒性增强是由于Ag NPs的表面氧化和物理化学改性以及Ag+的脱落，导致银的生物利用度增加。我们的观察，太阳光可以诱导的TiO 2和银纳米粒子的物理化学转化，并提高其潜在的毒性强调需要进行未来的毒性研究，在环境相关的暴露条件下，以指导决策的安全处理的纳米粒子。
The increasing use of silver (Ag) and titanium dioxide (TiO2) nanoparticles (NPs) in consumer products and their inevitable seepage into the environment prompted us to investigate their potential toxicity to a fish cell line (BF-2) and zebrafish embryos under dark and Simulated Solar Light (SSL) exposure conditions. Using high throughput screening (HTS) platforms, we showed that the oxidative stress-dependent cytotoxicity and embryonic toxicity of NPs were significantly increased upon exposure to SSL. While, the toxicity of TiO2 NPs under SSL exposure could be explained by hydroxyl radical generation, the enhanced toxicity of Ag NPs under SSL exposure was due to surface oxidation and physicochemical modification of Ag NPs and shedding of Ag+, leading to an increased bioavailability of silver. Our observations that solar light could induce physicochemical transformation of TiO2 and Ag NPs and enhance their toxic potential emphasizes the need for conducting future toxicity studies under environmentally relevant exposure conditions to guide decision making on the safe handling of NPs.