Low-molecular-weight peptides derived from extracellular matrix as chemoattractants for primary endothelial cells
Low-molecular-weight peptides derived from extracellular matrix as chemoattractants for primary endothelial cells
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DOI：
10.1080/10623320490512390
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Badylak, SF
中科院分区：
文献类型：
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作者：
Li, F;Li, W;Badylak, SF
用于组织工程应用的合成和天然支架的发展包括促进特定细胞类型的附着，控制支架降解速率，促进血管生成或以其他方式调节宿主反应的策略。我们已经报道了从猪小肠粘膜下层（SIS）中提取的生物支架促进组织的建设性重塑，并在急性炎症期消退后长时间内招募骨髓源性细胞。我们还没有确定哪些细胞被招募，这些细胞的最终命运，或者这些事件通过什么机制发生。我们现在通过功能和形态学方法分析了酸水解SIS的不同分子量组分，并确定了组分4（5至16 kDa）在体外对原代小鼠成年肝脏、心脏和肾脏内皮细胞具有化学引诱活性。将Matrigel栓子皮下植入小鼠体内后，添加分数4可促进体内血管形成。这些结果表明，从猪SIS降解中获得的小分子肽在体外和体内对小鼠内皮细胞的募集具有生物活性。
The development of synthetic and naturally occurring scaffolds for tissue engineering applications has included strategies to promote attachment of specific cell types, control the rate of scaffold degradation, encourage angiogenesis, or otherwise modulate the host response. We have reported that bioscaffolds developed from porcine small intestinal submucosa (SIS) facilitate the constructive remodeling of tissues and recruit marrow-derived cells that persist long after the acute inflammatory stages have resolved. We have not yet determined which cells are recruited, the eventual fate of these cells, or via what mechanisms the events occur. We now have analyzed various molecular weight fractions of acid-hydrolyzed SIS by both functional and morphologic methods and have determined that fraction 4 (5 to 16 kDa) possesses chemoattractant activity for primary murine adult liver, heart, and kidney endothelial cells in vitro. Addition of fraction 4 to Matrigel plugs promoted in vivo vascularization when the plugs were implanted subcutaneously in mice. These results indicate that small-molecular-weight peptides derived from the degradation of porcine SIS are biologically active in the recruitment of murine endothelial cells in vitro and in vivo.