LEFPS1, a tomato farnesyl pyrophosphate gene highly expressed during early fruit development
LEFPS1, a tomato farnesyl pyrophosphate gene highly expressed during early fruit development
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DOI：
10.1104/pp.123.4.1351
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发表时间：
2000-08-01
期刊：
影响因子：
7.4
通讯作者：
Gallusci, P
中科院分区：
文献类型：
--
作者：
Gaffe, J;Bru, JP;Gallusci, P
法尼基焦磷酸合酶 (FPS) 催化法尼基焦磷酸的合成，法尼基焦磷酸是甾醇和倍半萜生物合成的关键中间体。使用基于聚合酶链反应的方法，我们对 LeFPS1 进行了表征，LeFPS1 是一种番茄 (Lycoperscion esculentum cv Wva 106) 果实 cDNA，它编码功能性 FPS。我们证明番茄 FPS 由一个小型多基因家族编码，其基因位于 10 号和 12 号染色体上。与甾醇生物合成中法呢基焦磷酸的需求一致，FPS 基因在番茄植株中普遍表达。使用 LeFPS1 特异性探针，我们发现相应的基因可以解释大多数植物器官中的大部分 FPS mRNA，但在幼苗发育过程中则不然，这表明番茄中 FPS 基因的差异调节。 FPS基因表达也受到严格的发育控制：FPS mRNA主要在年轻器官中丰富，并随着器官成熟而减少，但果实呈现双相积累模式。在后一种情况下，原位杂交研究表明 FPS mRNA 在幼果的所有组织中同样丰富。综上所述，我们的结果表明，几种 FPS 亚型参与番茄法尼基焦磷酸代谢，并且 FPS 基因主要表达与细胞分裂和增大有关。
Farnesyl pyrophosphate synthase (FPS) catalyzes the synthesis of farnesyl pyrophosphate, a key intermediate in sterol and sesquiterpene biosynthesis. Using a polymerase chain reaction-based approach, we have characterized LeFPS1, a tomato (Lycoperscion esculentum cv Wva 106) fruit cDNA, which encodes a functional FPS. We demonstrate that tomato FPSs are encoded by a small multigenic family with genes located on chromosomes 10 and 12. Consistent with farnesyl pyrophosphate requirement in sterol biosynthesis, FPS genes are ubiquitously expressed in tomato plants. Using an LeFPS1 specific probe, we show that the corresponding gene can account for most of FPS mRNA in most plant organs, but not during young seedling development, indicating a differential regulation of FPS genes in tomato. FPS gene expression is also under strict developmental control: FPS mRNA was mainly abundant in young organs and decreased as organs matured with the exception of fruits that presented a biphasic accumulation pattern. In this latter case in situ hybridization studies have shown that FPS mRNA is similarly abundant in all tissues of young fruit. Taken together our results suggest that several FPS isoforms are involved in tomato farnesyl pyrophosphate metabolism and that FPS genes are mostly expressed in relation to cell division and enlargement.