Probing Calcium Ions with Biosensors
Probing Calcium Ions with Biosensors
复制标题
用生物传感器探测钙离子
DOI：
10.1080/02648725.2003.10648035
复制
发表时间：
2003
影响因子：
--
通讯作者：
Masamichi Ohkura
中科院分区：
文献类型：
--
作者：
J. Nakai;Masamichi Ohkura
众所周知，钙离子（Ca+）在细胞功能中起重要作用。如肌丝收缩、分泌、纤维素酶活性和膜电位等。因此，科学家们测量了细胞内Ca 2+浓度（[Ca 2 +]）。目前已发展了几种测定[Ca+]的方法，包括离子选择性电极法、Ca ~（2+）测定法和离子选择性电极法。指示染料和生物传感器。除此之外，现在最流行的是使用Ca+指示剂染料，例如在20世纪80年代早期开发的fura-2（Tsien等，1982），因为应用乙酰氧基甲基形式的Ca 2+指示剂染料使得染料更容易装载到细胞中，并且因为通过使用Ca+指示剂染料使得Ca+成像成为可能。Ca2 ~.指示染料确实是生物学上的一个重大进步。然而，这些Ca 2+指示染料有时不能满足某些特定的科学需要。例如，将Ca ~（2+）指示染料定位于特定的细胞内细胞器并不容易，相反，有时会观察到染料在细胞和细胞器中的意外积累。此外，Ca 2+指示剂染料可以从细胞中挤出。最后，除了一些特殊情况外，不可能实现Ca+指示染料的细胞类型特定负载。由于从水母Aequorea维多利亚克隆了绿色荧光蛋白（OFP）（Prasher等人，1992），它已被广泛应用于生物领域作为标记或报告蛋白。此外，现在可以制作几种不同类型的含有GFP的Ca 2+探针（生物传感器）。由于这些Ca 2+探针是遗传编码的，因此可以在特定细胞或细胞内表达探针。
It is well known that calcium ions (Ca") play important roles in cellular functions., such as Inuscle contraction, secretion, and controllingenzylne activities and menlbrane potentials. Hence, scientists have Ineasured intracellular Ca2+ concentrations ([Ca2+]) in cells. To date, severallnethods have been developed in order to Ineasure [Ca+], which include ion-selective electrodes, Ca... indicator dyes, and biosensors. Alnongst them, it is now most popular to use Ca+ indicator dyes such as fura-2, which were developed in the early 1980s (Tsien et ol.t 1982), because application of acetoxy methyl forms of Ca2+ indicator dyes makes loading of the dyes to cells much easier, and because Ca+ imaging is made possible by using Ca+ indicator dyes. The development ofCa2~. indicatordyes is indeed one of the big steps in biology. However, these Ca2+ indicator dyes occasionally cannot satisfy some particular scientific needs. For exanlple, it is not easy to target Ca2+ indicator dyes to specific intracellular organelles, orconversely, unexpected accumulations ofthe dyes to cells and organelles are occasionally observed. In addition, Ca2+ indicator dyes can be extruded from cells. Finally, cell-type specific loadings of Ca+ indicator dyes are not possible, except for some special cases. Since green fluorescent protein (OFP) was cloned from the jellyfish Aequorea victoria (Prasher et a!., 1992), it has been applied intensively in the biological field as a marker or a reporter protein. In addition, it is now possible to make several different types of Ca2+ probes (biosensors) containing GFP. Because these Ca2+ probes are genetically encoded, it becomes possible to express the probes in specific cells or
影响因子：
4
作者：
Persechini, A;Lynch, JA;Romoser, VA
通讯作者：
Romoser, VA