CYTOPLASMIC PHASES IN THE 1ST CELL-CYCLE OF THE ACTIVATED FROG EGG
CYTOPLASMIC PHASES IN THE 1ST CELL-CYCLE OF THE ACTIVATED FROG EGG
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DOI：
10.1016/0012-1606(83)90237-3
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
ELINSON, RP
中科院分区：
文献类型：
--
作者：
ELINSON, RP
激活的青蛙卵的第一个细胞周期比随后的周期长，此时发生了几个发育上重要的事件，如两侧对称性的确定。在激活后的不同时间解剖淡色对虾或非洲爪哇的卵，可以直观地检测到动物半细胞质的一致性差异，并在此基础上将第1个细胞周期划分为4个细胞质时相。第1阶段包括激活事件，持续约1/3的第1周期。在第二时相，细胞质变得液体，除了坚硬的、正在生长的精子外，原核的大部分迁移发生在第二时相。在第三相，无论是否有精子原，细胞质都变得坚硬，灰色新月形，表明两侧对称的平面。细胞质的坚固性对秋水仙素敏感，但细胞松弛素B不敏感，灰色新月体的形成也是如此。在第四阶段，皮质脱离坚固的细胞质，现在坚固对细胞松弛素B敏感，对秋水仙素不敏感。在第1个细胞周期中，细胞质一致性的变化可能反映了细胞骨架的变化，并且细胞质一致性与第1个细胞周期中的发育事件有关。
The 1st cell cycle of the activated frog egg is longer than subsequent cycles and several developmentally important events, such as the determination of bilateral symmetry, occur at this time. When eggs of R. pipiens or Xenopus laevis are dissected at times after activation, differences in the consistency of the animal half cytoplasm can be detected visually, and the 1st cell cycle was divided into 4 cytoplasmic phases on this basis. Phase 1 includes the events of activation and lasts about 1/3 of the 1st cycle. In phase 2 the cytoplasm becomes fluid except for the rigid, growing sperm aster, and most of the migration of the pronuclei occurs in phase 2. In phase 3 the cytoplasm becomes firm whether or not a sperm aster had been present, and the grey crescent forms, indicating the plane of bilateral symmetry. The firmness of the cytoplasm is colchicine sensitive but cytochalasin B insensitive as is grey crescent formation. In phase 4 the cortex detaches from the firm cytoplasm, and the firmness is now cytochalasin B sensitive and colchicine insensitive. Changes in cytoplasmic consistency during the 1st cell cycle probably reflect changes in the cytoskeleton, and the cytoplasmic consistency is functionally correlated with developmental events in the 1st cell cycle.