Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism
Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism
复制标题
DOI：
10.1111/j.1600-0722.2011.00817.x
复制
发表时间：
2011-04-01
影响因子：
1.9
通讯作者：
Russell, Roy R. B.
中科院分区：
文献类型：
--
作者：
Izutani, Naomi;Imazato, Satoshi;Russell, Roy R. B.
抗菌单体 12-甲基丙烯酰氧基十二烷基溴化吡啶 (MDPB) 在未聚合时是一种强杀菌剂，具有在各种树脂生物材料中应用的潜力。为了详细分析该单体的抗菌特性，研究了高浓度未聚合MDPB在短时间内杀死浮游或生物膜形式的变形链球菌的能力，以及低浓度MDPB对变形链球菌代谢功能的抑制作用。高浓度的MDPB可在60秒内有效杀死浮游和生物膜变形链球菌细胞，并且通过与1,000μg ml-1的MDPB接触60秒获得完全杀死。当细菌数量较低时，MDPB 的浓度为 4-8 mu g ml-1，表现出生长抑制作用，诱导滞后期和倍增时间延长。 8μgml-1的MDPB对变形链球菌产酸的抑制可能是由于乳酸脱氢酶活性的抑制所致。在高浓度下，MDPB 在短时间内对浮游和生物膜形式的变形链球菌具有致命性，而在低浓度下，MDPB 会抑制代谢酶活性。
The antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) is a strong bactericide when unpolymerized and has the potential to be utilized in various resinous biomaterials. To analyze the antibacterial characteristics of this monomer in detail, the ability of high concentrations of unpolymerized MDPB to kill Streptococcus mutans in planktonic or biofilm forms within a short time-period of contact, and the inhibitory effects of low concentrations of MDPB on the metabolic function of S. mutans, were examined. High concentrations of MDPB showed effective killing of planktonic and biofilm S. mutans cells within 60 s, and complete killing was obtained by contact with 1,000 mu g ml-1 of MDPB for 60 s. At a concentration of 4-8 mu g ml-1, MDPB demonstrated growth inhibition, inducing elongation of the lag phase and of the doubling time, when the bacterial number was low. Inhibition of the production of acid from S. mutans by 8 mu g ml-1 of MDPB may have been caused by the inhibition of lactate dehydrogenase activity. At high concentrations, MDPB is lethal to both planktonic and biofilm forms of S. mutans in a short time-period, and at low concentrations, MDPB inhibits metabolic enzymatic activity.