Solution NMR investigations of integral membrane proteins: Challenges and innovations
Solution NMR investigations of integral membrane proteins: Challenges and innovations
复制标题
DOI：
10.1016/j.sbi.2023.102654
复制
发表时间：
2023-08-03
影响因子：
6.8
通讯作者：
Columbus，Linda
中科院分区：
文献类型：
--
作者：
Necelis，Matthew;Mcdermott，Connor;Columbus，Linda
与可溶性蛋白质相比，膜蛋白的稳定性、溶剂相互作用和功能的理解还不清楚。膜蛋白的标记，表达和稳定化的最新进展，使溶液核磁共振光谱研究膜蛋白的构象状态，配体结合，脂质相互作用，稳定性和折叠。这篇综述强调了这些进展和新的理解，并提供了最近的应用程序的例子。
Compared to soluble protein counterparts, the understanding of membrane protein stability, solvent interactions, and function are not as well understood. Recent advancements in labeling, expression, and stabilization of membrane proteins have enabled solution nuclear magnetic resonance spectroscopy to investigate membrane protein conformational states, ligand binding, lipid interactions, stability, and folding. This review highlights these advancements and new understandings and provides examples of recent applications.