Passive anti-PcrV treatment protects burned mice against Pseudomonas aeruginosa challenge
Passive anti-PcrV treatment protects burned mice against Pseudomonas aeruginosa challenge
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DOI：
10.1016/j.burns.2004.09.002
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发表时间：
2005-03-01
期刊：
影响因子：
2.7
通讯作者：
Sawa, T
中科院分区：
文献类型：
--
作者：
Neely, AN;Holder, IA;Sawa, T
III型分泌系统包括分泌的外源产物和结构成分，如PcrV，该系统在烧伤宿主中对铜绿假单胞菌的毒力起着重要作用。本研究的目的是确定被动抗PERV治疗是否能保护烧伤小鼠免受致死性铜绿假单胞菌的攻击，并确定作为攻击菌株的铜绿假单胞菌的III型外源产物表型。在兔体内制备抗血清。小鼠被三度烧伤，用致死剂量的铜绿假单胞菌攻击，并用抗PcrV或对照免疫球蛋白腹腔注射治疗。测试了对三种不同假单胞菌的保护作用。聚合酶链式反应基因分型和免疫印迹分型结果表明，所有铜绿假单胞菌均为侵袭型III型：ExOS+和/或ExoT+、ExoU-、ExoY+。对于所有毒株，抗PcrV治疗组的存活率显著高于对照免疫球蛋白治疗组(P<；0.05)。在烧伤后24小时再次注射PcrV抗血清可延长显著保护持续时间。因此，被动抗PcrV免疫可以保护烧伤小鼠免受侵袭性III型铜绿假单胞菌的致命攻击。这种免疫疗法可能被进一步探索为治疗烧伤宿主中高度耐药的铜绿假单胞菌感染的方法。(C)2004年爱思唯尔有限公司和ISBI。版权所有。
The type III secretion system consists of secreted exoproducts and structural components, such as PcrV, and this system plays an important role in the virulence of Pseudomonas aeruginosa in burned hosts. The purpose of this study was to determine if passive anti-PerV treatment would protect burned mice from fatal P. aeruginosa challenge, and to determine the type III exoproduct phenotype of the P. aeruginosa used as challenge strains. Antiserum was raised in rabbits. Mice were given a third degree burn, challenged with a lethal dose of R aeruginosa, and treated with either anti-PcrV or control immunoglobulin intraperitoneally. Protection against three different pseudomonads was tested. Genotyping by PCR and phenotyping by immunoblots showed the P. aeruginosa strains to all be of the invasive type III phenotype: ExoS+ and/or ExoT+, ExoU-, ExoY+. Against all strains, the anti-PcrV treatment yielded significantly better survival (P < 0.05) than the control immunoglobulin treatment. Duration of significant protection was improved by giving a second injection of PcrV antisera at 24 h postburn. Hence, passive anti-PcrV immunization could protect burned mice against fatal challenge with P. aeruginosa of an invasive type III phenotype. This immunotherapy might be explored further as possible treatment for highly antibiotic resistant P. aeruginosa infections in burned hosts. (C) 2004 Elsevier Ltd and ISBI. All rights reserved.