Monoclonal antibody specific to acid phosphatase isoenzyme 4
Monoclonal antibody specific to acid phosphatase isoenzyme 4
复制标题
酸性磷酸酶同工酶 4 特异性单克隆抗体
DOI：
10.1002/pros.2990090108
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
L. Yam
中科院分区：
文献类型：
--
作者：
Howard Dang;K. Lam;chin;L. Wold;Tsieh Sun;L. Yam
前列腺酸性磷酸酶同工酶4经离子交换柱层析、高压液相色谱分离纯化。用该高纯度的酶免疫BALB/c小鼠，制备单抗。该抗体对同工酶4具有特异性，对同工酶2几乎没有亲和力。用Western印迹分析和放射免疫抑制法评价了该单抗的特异性。
Prostatic acid phosphatase isoenzyme 4 was purified by ion exchange column chromatography, followed by high pressure liquid chromatography. The highly purified enzyme was used to produce monoclonal antibody from immunized BALB/c mice. The antibody was specific to isoenzyme 4, with negligible affinity to isoenzyme 2. The specificity of the monoclonal antibody was evaluated by Western blot analysis and by inhibition of radioimmunoassay.