Enhanced production of longer side-chain polyhydroxyalkanoic acid with ω-aromatic group substitution in phaZ-disrupted Pseudomonas fluorescens BM07 mutant through unrelated carbon source cometabolism and salicylic acid β-oxidation inhibition
Enhanced production of longer side-chain polyhydroxyalkanoic acid with ω-aromatic group substitution in phaZ-disrupted Pseudomonas fluorescens BM07 mutant through unrelated carbon source cometabolism and salicylic acid β-oxidation inhibition
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DOI：
10.1016/j.biortech.2010.01.082
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发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Yoon, Sung Chul
中科院分区：
文献类型：
--
作者：
Choi, Mun Hwan;Xu, Ju;Yoon, Sung Chul
荧光假单胞菌 BM07 中胞内聚羟基脂肪酸酯 (PHA) 解聚酶基因 (phaZ) 的缺失被发现比野生型菌株更有效地增加了较长中链长度 (MCL) omega-芳香族单体单元的水平，当细胞与果糖和 MCL omega-芳香族脂肪酸的混合物一起在水杨酸存在下生长时，水杨酸被称为 β-氧化抑制剂。 BM07菌株。当使用11-苯氧基十一烷酸作为共碳源时，文献中未报道的最长单体单元3-羟基-11-苯氧基十一烷酸酯被掺入到聚合物链中，含量高达大约10 mol%。水杨酸抑制技术的优点是水杨酸在BM07菌株中不被代谢，因此，抑制剂的有效浓度在整个培养过程中保持恒定。总之，这项新技术可用于增强侧链调节功能性 MCL-PHA 的生产，并改善荧光假单胞菌 BM07 的理化性质。 (C) 2010 Elsevier Ltd. 保留所有权利。
The deletion of the intracellular polyhydroxyalkanoate (PHA) depolymerase gene (phaZ) in Pseudomonas fluorescens BM07 was found to increase more efficiently the levels of longer medium-chain-length (MCL) omega-aromatic monomer-units than in the wild-type strain when the cells were grown with a mixture of fructose and MCL omega-aromatic fatty acid in the presence of salicylic acid that is known as a beta-oxidation inhibitor in BM07 strain. When 11-phenoxyundecanoic acid was used as co-carbon source, the longest monomer-unit 3-hydroxy-11-phenoxyundecanoate, not reported in literature yet, was incorporated into the polymer chain up to similar to 10 mol%. An advantage of salicylic acid inhibition technique is that salicylic acid is not metabolized in BM07 strain, thus, the effective concentration of the inhibitor remaining constant throughout the cultivation. In conclusion, this new technique could be exploited for the enhanced production of side-chain modulated functional MCL-PHA with improved physicochemical properties in P. fluorescens BM07. (C) 2010 Elsevier Ltd. All rights reserved.