REDUCED THYMIC MATURATION BUT NORMAL EFFECTOR FUNCTION OF CD8+ T-CELLS IN CD8-BETA GENE-TARGETED MICE
REDUCED THYMIC MATURATION BUT NORMAL EFFECTOR FUNCTION OF CD8+ T-CELLS IN CD8-BETA GENE-TARGETED MICE
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DOI：
10.1084/jem.180.3.959
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发表时间：
1994-09-01
影响因子：
15.3
通讯作者：
LAU, CY
中科院分区：
文献类型：
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作者：
FUNGLEUNG, WP;KUNDIG, TM;LAU, CY
CD 8是主要组织相容性复合体I类限制性T细胞上的细胞表面糖蛋白。胸腺细胞和大多数外周T细胞将CD 8表达为CD 8 α和CD 8 β的异源二聚体。肠上皮内淋巴细胞（IEL），已被认为是胸腺外产生的，主要表达CD 8作为CD 8 α的同源二聚体。我们已经产生了CD 8 β基因靶向小鼠。胸腺和大多数外周淋巴器官中的CD 8 α（+）T细胞群减少至野生型同窝仔的20-30%。胸腺细胞和外周T细胞上的CD 8 α表达也分别下降至正常水平的44%和53%。相反，CD 8 α（+）IEL的群体大小和CD 8 α表达水平均未降低。这一发现表明，CD 8 β仅对胸腺来源的CD 8（+）T细胞很重要。缺乏CD 8 β会降低但不会完全消除CD 8（+）T细胞的胸腺成熟。我们的结果还揭示了CD 8 β在调节胸腺衍生T细胞上的CD 8 α表达中的作用。这些小鼠的外周血T细胞对淋巴细胞性脉络丛脑膜炎病毒和水泡性口炎病毒具有有效的细胞毒活性，表明CD 8 β对CD 8（+）T细胞的效应功能不是必需的。
CD8 is a cell surface glycoprotein on major histocompatibility complex class I-restricted T cells. Thymocytes and most peripheral T cells express CD8 as heterodimers of CD8 alpha and CD8 beta. The intestinal intraepithelial lymphocytes (IEL), which have been suggested to be generated extrathymically, express CD8 predominantly as homodimers of CD8 alpha. We have generated CD8 beta gene-targeted mice. CD8 alpha(+) T cell population in the thymus and in most peripheral lymphoid organs was reduced to 20-30% of that in wild-type littermates. CD8 alpha expression on thymocytes and peripheral T cells also decreased to 44 and 53% of the normal levels, respectively. In contrast, neither the population size nor the CD8 alpha expression level of CD8 alpha(+) IEL was reduced. This finding indicates that CD8 beta is important only for thymic-derived CD8(+) T cells. The lack of CD8 beta reduces but does not completely abolish thymic maturation of CD8(+) T cells. Our result also reveals the role of CD8 beta in regulating CD8 alpha expression on thymic derived T cells. Peripheral T cells in these mice were efficient in cytotoxic activity against lymphocytic choriomeningitis virus and vesicular stomatitis virus, suggesting that CD8 beta is not essential for the effector function of CD8(+) T cells.