HYPER-RECOMBINATION IN UVRD MUTANTS OF ESCHERICHIA-COLI-K-12
HYPER-RECOMBINATION IN UVRD MUTANTS OF ESCHERICHIA-COLI-K-12
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DOI：
10.1007/bf00267368
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
LLOYD, RG
中科院分区：
文献类型：
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作者：
ARTHUR, HM;LLOYD, RG
最初由于其强超重组表型而分离出的大肠杆菌突变株携带 uvrD 损伤。这种名为 uvrD210 的突变的存在增加了 F-prime 复制细胞中染色体重复之间的重组频率，并减少了与 Hfr 供体杂交中紧密连锁标记之间的连锁。在携带 uvrD 的其他等位基因（以前称为 mutU4、uvr502 和 recL152）的菌株中证实了类似的 hyper-rec 表型。 uvrD210菌株的重组活性因recA突变而被消除，但与该等位基因相关的突变活性被证明与recA无关。 UvrD 突变降低了 DNA 复制的保真度，新合成链中损伤的积累为启动重组提供了额外的位点。
A mutant strain of E. coli which was isolated initially because of its strong hyper-recombination phenotype carried a lesion in uvrD. The presence of this mutation, designated uvrD210, increased the frequency of recombination between chromosomal duplications in F-prime repliconant cells and reduced linkage between closely linked markers in crosses with Hfr donors. A comparable hyper-rec phenotype was demonstrated in strains carrying other alleles of uvrD previously referred to as mutU4, uvr502 and recL152. The recombination activity of a uvrD210 strain was abolished by mutation of recA but the mutator activity associated with this allele proved to be independent of recA. UvrD mutations reduce the fidelity of DNA replication and the accumulation of lesions in the newly synthesized strand provides additional sites for initiating recombination.