Determination of mercurial species in fish by inductively coupled plasma mass spectrometry with anion exchange chromatographic separation
Determination of mercurial species in fish by inductively coupled plasma mass spectrometry with anion exchange chromatographic separation
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阴离子交换色谱分离电感耦合等离子体质谱法测定鱼类中的汞种类
DOI：
10.1016/j.aca.2013.08.017
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发表时间：
2013-09-24
影响因子：
6.2
通讯作者：
Yin, Xuefeng
中科院分区：
文献类型：
--
作者：
Chen, Xiaopan;Han, Chao;Yin, Xuefeng
本工作证明了负离子交换色谱分离-电感耦合等离子体质谱检测汞形态分析的可行性。首次通过与含3-巯基-1-丙磺酸的流动相络合成带负电荷的配合物，在12.5 mm强阴离子交换柱上，在5 min内实现了无机汞（Hg2+）、单甲基汞（MeHg）、乙基汞（EtHg）和苯汞（PhHg）的快速分离。Hg2+、MeHg、EtHg和PhHg的检出限分别为0.008、0.024、0.029和0.034 μ g -1。峰高和峰面积的相对标准偏差（各汞种5.0 μ g L-1）均小于3%。该方法测定的鱼组织标准物质中Hg2+、MeHg和总Hg的含量与认证值吻合良好，回收率满意。测定甲基汞和总汞的相对误差分别为-2.4%和-1.2%，峰回收率可接受范围为94-101%。然后在预处理程序后分析了11个鱼样品中的汞形态。所有鱼类样本的汞含量均符合中国国家标准。(C) 2013 Elsevier B.V.版权所有
This work demonstrated the feasibility of mercury speciation analysis by anion exchange chromatographic separation with inductively coupled plasma mass spectrometry detection. For the first time, by complexing with the mobile phase containing 3-mercapto-1-propanesulfonate into negatively charged complexes, fast separation of inorganic mercury (Hg2+), monomethylmercury (MeHg), ethylmercury (EtHg) and phenylmercury (PhHg) was achieved within 5 min on a 12.5-mm strong anion exchange column. The detection limits for Hg2+, MeHg, EtHg and PhHg were 0.008, 0.024, 0.029 and 0.034 mu g L-1, respectively. The relative standard deviations of peak height and peak area (5.0 mu g L-1 for each Hg species) were all below 3%. The determined contents of Hg2+, MeHg and total Hg in a certified reference material of fish tissue by the proposed method were in good accordance with the certified values with satisfactory recoveries. The relative errors for determining MeHg and total mercury were -2.4% and -1.2%, respectively, with an acceptable range for spike recoveries of 94-101%. Mercury speciation in 11 fish samples were then analyzed after the pretreated procedure. The mercury contents in all fish samples analyzed were found compliant with the criteria of the National Standards of China. (C) 2013 Elsevier B.V. All rights reserved.