Spectroscopic characterization and the pH dependence of bactericidal activity of the aqueous chlorine solution
Spectroscopic characterization and the pH dependence of bactericidal activity of the aqueous chlorine solution
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DOI：
10.2116/analsci.14.691
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发表时间：
1998-08-01
影响因子：
1.6
通讯作者：
Arata, Y
中科院分区：
文献类型：
--
作者：
Nakagawara, S;Goto, T;Arata, Y
拉曼光谱分析已被用于鉴别氯水溶液中的化学物质。得到的拉曼光谱的pH依赖性表明次氯酸离子、次氯酸和氯之间存在平衡。采用半定量生物测定法，对NaCl水溶液在pH 2 ~ 9范围内的酸性电解水对大肠杆菌K12和枯草芽孢杆菌PCI219的杀菌活性进行了评价。两种细菌在pH值为4 ~ 5时活性最高。利用拉曼光谱和紫外光谱数据，以及化学分析数据，得出了杀菌活性与溶液中次氯酸浓度定量相关的结论。
Raman spectroscopic analysis has been used to identify the chemical species that exist in aqueous chlorine solution. The pH dependence of the Raman spectra obtained indicates that there :is an equilibrium among hypochlorite ion, hypochlorous acid and chlorine. Bactericidal activities of the acidic electrolyzed water, which is generated by electrolysis of an aqueous NaCl solution, were evaluated in the pH range 2-9 against Escherichia coli K12 and Bacillus subtilis PCI219 by a semi-quantitative bioassay. The maximum activity was observed between pH 4 and 5 in both bacteria. The Raman and the ultraviolet spectroscopic data, along with chemical analysis data, were used to conclude that the bactericidal activity is quantitatively correlated to the concentration of hypochlorous acid in solution.