ANTIOXIDANT AND PROOXIDANT PROPERTIES OF ACTIVE ROSEMARY CONSTITUENTS - CARNOSOL AND CARNOSIC ACID
ANTIOXIDANT AND PROOXIDANT PROPERTIES OF ACTIVE ROSEMARY CONSTITUENTS - CARNOSOL AND CARNOSIC ACID
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DOI：
10.3109/00498259209046624
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发表时间：
1992-02-01
期刊：
影响因子：
1.8
通讯作者：
LOLIGERS, J
中科院分区：
文献类型：
--
作者：
ARUOMA, OI;HALLIWELL, B;LOLIGERS, J
1. 鼠尾草酚和鼠尾草酸被认为占迷迭香提取物抗氧化性能的90%以上。 纯化的鼠尾草酚和鼠尾草酸是微粒体和脂质体系统中强有力的脂质过氧化抑制剂，比没食子酸丙酯更有效. 鼠尾草酚和鼠尾草酸是很好的过氧化氢自由基（CCl_3O_2）清除剂。通过脉冲辐解产生，计算的速率常数分别为1-3 x 10（6）M-1 s-1和2.7 x 10（7）M-1 s-1。 鼠尾草酸与HOCl反应以保护蛋白质α（1）-抗蛋白酶免于失活。 鼠尾草酚和鼠尾草酸在博莱霉素试验中刺激DNA损伤，但在脱氧核糖试验中清除羟基自由基。 在脱氧核糖体系中，鼠尾草酚和鼠尾草酸与.OH反应的计算速率常数分别为8.7 x 10（10）M-1 s-1和5.9 x 10（10）M-1 s-1。 鼠尾草酸似乎是过氧化氢，但它也可以作为过氧化物酶系统的底物。 鼠尾草酸和鼠尾草酚还原细胞色素c，但速率常数显著低于O2-。
1. Carnosol and carnosic acid have been suggested to account for over 90% of the antioxidant properties of rosemary extract.2. Purified carnosol and carnosic acid are powerful inhibitors of lipid peroxidation in microsomal and liposomal systems, more effective than propyl gallate.3. Carnosol and carnosic acid are good scavengers of peroxyl radicals (CCl3O2.) generated by pulse radiolysis, with calculated rate constants of 1-3 x 10(6) M-1 s-1 and 2.7 x 10(7) M-1 s-1 respectively.4. Carnosic acid reacted with HOCl in such a way as to protect the protein alpha(1)-antiproteinase against inactivation.5. Both carnosol and carnosic acid stimulated DNA damage in the bleomycin assay but they scavenged hydroxyl radicals in the deoxyribose assay. The calculated rate constants for reaction with .OH in the deoxyribose system for carnosol and carnosic acid were 8.7 x 10(10) M-1 s-1 and 5.9 x 10(10) M-1 s-1 respectively.6. Carnosic acid appears to scavenge H2O2, but it could also act as a substrate for the peroxidase system.7. Carnosic acid and carnosol reduce cytochrome c but with a rate constant significantly lower than that of O2-..