An external ion microbeam for studies of biological samples
An external ion microbeam for studies of biological samples
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用于生物样品研究的外部离子微束
DOI：
10.1007/bf02784193
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发表时间：
2007
影响因子：
3.9
通讯作者：
K. Ishii
中科院分区：
文献类型：
--
作者：
T. Sakai;Yutaka Naitoh;T. Kamiya;S. Matsuyama;K. Gotoh;Satoshi Yotoka;Hiromichi Yamasaki;K. Ishii
日本原子力研究所高崎研制了一种新的外离子束系统，并将其与轻离子微束系统相结合。该系统设计用于空气环境中生物样品的微粒子诱导X射线发射分析，空间分辨率为1 Μm。保持如此高的空间分辨率的最严重的问题之一是在光束出射窗口的多次散射。采用薄Kapton膜（7.5 Μm厚）作为出射窗以及样品背衬箔以使膜和样品之间的距离最小化。研究了薄膜在离子辐照下的寿命和外部微束的空间分辨率。结果表明，该膜能承受足够长时间的辐照，并能保持近1 Μm的分辨率。
A new external ion-beam system was developed and combined with a light ion-microbeam system in JAERI Takasaki. The system is designed for micro-particle-induced X-ray emission analysis of biological samples in air environment with 1 Μm spatial resolution. One of the most serious problems in keeping such a high spatial resolution is multiple scattering in a beam exit window. Thin Kapton film (7.5 Μm thick) was adopted as the exit window as well as a sample backing foil to minimize the distance between the film and samples. The lifetime of the foil under ion irradiation and spatial resolution of the external microbeam were investigated. The results shows that the film can endure sufficient long-time irradiation to take elemental maps and the resolution can be kept nearly 1 Μm.