The Decreased apical dominance 1/petunia hybrida carotenoid cleavage dioxygenase8 gene affects branch production and plays a role in leaf senescence, root growth, and flower development
The Decreased apical dominance 1/petunia hybrida carotenoid cleavage dioxygenase8 gene affects branch production and plays a role in leaf senescence, root growth, and flower development
复制标题
DOI：
10.1105/tpc.104.027714
复制
发表时间：
2005-03-01
期刊：
影响因子：
11.6
通讯作者：
Klee, HJ
中科院分区：
文献类型：
--
作者：
Snowden, KC;Simkin, AJ;Klee, HJ
类胡萝卜素和类胡萝卜素裂解产物在植物发育中起着重要的作用。矮牵牛（Petunia hybrida）的减少顶端优势1（Dad 1）/PhCCD 8基因编码假定的类胡萝卜素裂解双加氧酶（CCD）和多轴生长4（MAX 4）/AtCCD 8基因的直系同源物。dad 1 -1突变等位基因通过插入不寻常的转座子（Dad-1转座子）而失活，并且dad 1 -3等位基因是dad 1 -1的回复突变等位基因。Dad 1/PhCCD 8基因在根和芽组织中表达，这与其在产生可改变分支的嫁接传递化合物中的作用一致。这种表达在dad 1 -1，dad 2和dad 3增加分支突变体的茎中上调，表明该基因在该组织中的反馈调节。然而，这种反馈调节并不影响Dad 1/PhCCD 8的根表达。在dad 1 -1突变体中Dad 1/PhCCD 8的过表达补充了突变体表型，而在野生型中的RNA干扰导致了分支表型的增加。与Dad 1/PhCCD 8功能丧失相关的表型的其他差异包括腋生分生组织发育的时间改变、叶片衰老延迟、花较小、节间长度减少和根生长减少。这些数据表明Dad 1/PhCCD 8酶的底物和/或产物是移动的信号分子，在植物发育中具有不同的作用。
Carotenoids and carotenoid cleavage products play an important and integral role in plant development. The Decreased apical dominance1 (Dad1)/PhCCD8 gene of petunia (Petunia hybrida) encodes a hypothetical carotenoid cleavage dioxygenase (CCD) and ortholog of the MORE AXILLARY GROWTH4 (MAX4)/AtCCD8 gene. The dad1-1 mutant allele was inactivated by insertion of an unusual transposon (Dad-one transposon), and the dad1-3 allele is a revertant allele of dad1-1. Consistent with its role in producing a graft-transmissible compound that can alter branching, the Dad1/PhCCD8 gene is expressed in root and shoot tissue. This expression is upregulated in the stems of the dad1-1, dad2, and dad3 increased branching mutants, indicating feedback regulation of the gene in this tissue. However, this feedback regulation does not affect the root expression of Dad1/PhCCD8. Overexpression of Dad1/PhCCD8 in the dad1-1 mutant complemented the mutant phenotype, and RNA interference in the wild type resulted in an increased branching phenotype. Other differences in phenotype associated with the loss of Dad1/PhCCD8 function included altered timing of axillary meristem development, delayed leaf senescence, smaller flowers, reduced internode length, and reduced root growth. These data indicate that the substrate(s) and/or product(s) of the Dad1/PhCCD8 enzyme are mobile signal molecules with diverse roles in plant development.