OsFPFL4 is Involved in the Root and Flower Development by Affecting Auxin Levels and ROS Accumulation in Rice (Oryza sativa)
OsFPFL4 is Involved in the Root and Flower Development by Affecting Auxin Levels and ROS Accumulation in Rice (Oryza sativa)
复制标题
OsFPFL4 通过影响水稻 (Oryza sativa) 中的生长素水平和 ROS 积累来参与根和花的发育
DOI：
10.1186/s12284-019-0364-0
复制
发表时间：
2020-01-07
期刊：
影响因子：
5.5
通讯作者：
Huang, Junli
中科院分区：
文献类型：
--
作者：
Guo, Yaomin;Wu, Qi;Huang, Junli
背景
FPF 1（flowing-promoting factor 1）是植物开花时间遗传调控的重要家族之一。到目前为止，对水稻FPF 1家族的了解有限。
结果
水稻FPF 1样蛋白4（OsFPFL 4）作为AtFPF 1的同源物，在植物的多种组织中表达。利用反向遗传学方法研究了OsFPFL 4在水稻中的功能。过量表达OsFPFL 4的植株与野生型相比，主根较短，侧根和不定根较多;但OsFPFL 4的RNA干扰（RNAi）显著抑制了根系的生长，并延迟了水稻开花时间。有趣的是，OsFPFL 4的表达增加或抑制导致花药萎缩和异常的花粉粒。生长素在植物根和花的发育中起着重要作用，根的伸长也受到活性氧稳态的调节。在这里，我们的结果表明，过表达OsFPFL 4的水稻植株在地上部和根部积累更多的生长素，而RNAi株系比野生型具有更少的生长素。正如预期的那样，在这些OsFPFL 4转基因植物中，负责生长素生物合成和极性运输的基因的转录水平被改变。对于ROS，在过表达的根和花序中检测到比野生型对应物略高的ROS水平;然而，在RNAi系中ROS水平显著增加，这是由于ROS生产者的表达增加和ROS清除剂的表达减少。
结论
结果表明，OsFPFL 4基因通过影响生长素和活性氧的稳态，参与调控水稻根和花的发育。OsFPFL 4通过影响生长素的合成和转运来调控生长素的积累，并通过平衡ROS的产生和清除来调节ROS的稳态。因此，生长素介导的ROS产生可能在调节氧化还原状态中起作用，该氧化还原状态控制植物根和花的发育。
Background
FPF1 (flowering-promoting factor 1) is one of the important family involved in the genetic control of flowering time in plant. Until now, limited knowledge concerning FPF1 family in rice has been understood.
Results
As a homologue of AtFPF1, FPF1-like protein 4 of rice (OsFPFL4) is expressed in various tissues of plants. The functions of OsFPFL4 in rice were investigated by the reverse genetics approaches. Plants overexpressing OsFPFL4 have shorter primary root, more lateral roots and adventitious roots than wild type; however, RNA interference (RNAi) of OsFPFL4 significantly inhibits the growth of root system, and also delays the flowering time in rice. Interestingly, increased or repressed expression of OsFPFL4 leads to shrunken anthers and abnormal pollen grains. It is well recognized that auxin plays important roles in plant root and flower development, and the root elongation is also regulated by reactive oxygen species (ROS) homeostasis. Here, our results show that rice plants overexpressing OsFPFL4 accumulate more auxin in the shoot and root, whereas RNAi lines have less auxin than wild type. As expected, the transcript levels of genes responsible for auxin biosynthesis and polar transport are altered in these OsFPFL4 transgenic plants. As to ROS, slightly higher ROS levels were detected in overexpression root and inflorescence than the counterparts of wild type; however, the ROS levels were significantly increased in the RNAi lines, due to increased expression of ROS-producers and reduced expression of ROS-scavengers.
Conclusion
Our results reveal that OsFPFL4 is involved in modulating the root and flower development by affecting auxin and ROS homeostasis in rice plants. OsFPFL4 controls auxin accumulation via affecting auxin biosynthesis and transport, and also modulates ROS homeostasis by balancing ROS producing and scavenging. Thus, auxin-mediated ROS production might play a role in regulating redox status, which controls plant root and flower development.