Tobacco rattle virus as a vector for analysis of gene function by silencing
Tobacco rattle virus as a vector for analysis of gene function by silencing
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DOI：
10.1046/j.0960-7412.2000.00942.x
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发表时间：
2001-01-01
期刊：
影响因子：
7.2
通讯作者：
Baulcombe, DC
中科院分区：
文献类型：
--
作者：
Ratcliff, F;Martin-Hernandez, AM;Baulcombe, DC
携带宿主衍生序列插入的病毒载体诱导受感染植物中相应基因的沉默。这种病毒诱导的基因沉默（VIGS）是RNA介导的防御机制的一种表现，与转基因植物中的转录后基因沉默（PTGS）相关。在此，我们描述了烟草脆裂病毒 (TRV) 的感染性 cDNA 克隆，该克隆已被修改以促进非病毒序列的插入和随后对植物的感染。我们证明，在没有病毒诱导症状的情况下，该载体介导内源基因的 VIGS。与之前用于 VIGS 的其他 RNA 病毒载体不同，TRV 构建体能够靶向植物生长点中的宿主 RNA。这些特征表明TRV载体将在植物基因发现方面具有广泛的应用。
Virus vectors carrying host-derived sequence inserts induce silencing of the corresponding genes in infected plants. This virus-induced gene silencing (VIGS) is a manifestation of an RNA-mediated defence mechanism that is related to post-transcriptional gene silencing (PTGS) in transgenic plants. Here we describe an infectious cDNA clone of tobacco rattle virus (TRV) that has been modified to facilitate insertion of non-viral sequence and subsequent infection to plants. We show that this vector mediates VIGS of endogenous genes in the absence of virus-induced symptoms. Unlike other RNA virus vectors that have been used previously for VIGS, the TRV construct is able to target host RNAs in the growing points of plants. These features indicate that the TRV vector will have wide application for gene discovery in plants.