Novel reductive dehalogenases from the marine sponge associated bacterium Desulfoluna spongiiphila
Novel reductive dehalogenases from the marine sponge associated bacterium Desulfoluna spongiiphila
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DOI：
10.1111/1758-2229.12556
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发表时间：
2017-10-01
影响因子：
3.3
通讯作者：
Haeggblom, Max M.
中科院分区：
文献类型：
--
作者：
Liu, Jie;Lopez, Nora;Haeggblom, Max M.
Desulfoluna spongiiiphila菌株AA 1是一种有机卤化物呼吸细菌，分离自海绵Aplysina aerophoba，除了硫酸盐和硫代硫酸盐作为末端电子受体外，还可以使用溴化和碘化苯酚。海绵脱硫叶菌菌株AA 1的基因组约为6.5 Mb。三个假定的还原脱卤酶（rdhA）基因参与呼吸代谢的有机卤化物的序列内确定。呼吸还原脱卤酶（一对精氨酸易位信号序列和两个铁硫簇）中发现的保守基序存在于所有三个假定的AA 1 rdhA基因。三个rdhA基因的转录显着上调呼吸过程中的2，6-二溴苯酚和海绵提取物。菌株AA 1似乎具有合成钴胺素的能力，钴胺素是大多数特征性还原脱卤酶的关键辅因子。基因组包含参与钴胺素合成和吸收的基因，并且可以在没有钴胺素补充的情况下生长。与脱溴相关的靶基因的鉴定为了解脱卤细菌如何控制海绵中有机卤化物的命运及其在共生有机溴循环中的作用奠定了基础。在海绵环境中，D.因此，嗜海绵菌菌株AA 1可以利用溴化化合物和硫酸盐作为呼吸的电子受体。
Desulfoluna spongiiphila strain AA1 is an organohalide respiring bacterium, isolated from the marine sponge Aplysina aerophoba, that can use brominated and iodinated phenols, in addition to sulfate and thiosulfate as terminal electron acceptors. The genome of Desulfoluna spongiiphila strain AA1 is approximately 6.5 Mb. Three putative reductive dehalogenase (rdhA) genes involved in respiratory metabolism of organohalides were identified within the sequence. Conserved motifs found in respiratory reductive dehalogenases (a twin arginine translocation signal sequence and two iron-sulfur clusters) were present in all three putative AA1 rdhA genes. Transcription of one of the three rdhA genes was significantly upregulated during respiration of 2,6-dibromophenol and sponge extracts. Strain AA1 appears to have the ability to synthesize cobalamin, the key cofactor of most characterized reductive dehalogenase enzymes. The genome contains genes involved in cobalamin synthesis and uptake and can grow without cobalamin supplementation. Identification of this target gene associated with debromination lays the foundation for understanding how dehalogenating bacteria control the fate of organohalide compounds in sponges and their role in a symbiotic organobromine cycle. Inthe sponge environment, D. spongiiphila strain AA1 may thus take advantage of both brominated compounds and sulfate as electron acceptors for respiration.