Molecular Detection of Zoonotic Rickettsiae and Anaplasma spp. in Domestic Dogs and Their Ectoparasites in Bushbuckridge, South Africa
Molecular Detection of Zoonotic Rickettsiae and Anaplasma spp. in Domestic Dogs and Their Ectoparasites in Bushbuckridge, South Africa
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DOI：
10.1089/vbz.2015.1849
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发表时间：
2016-04-01
影响因子：
2.1
通讯作者：
Matjila, Paul T.
中科院分区：
文献类型：
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作者：
Kolo, Agatha O.;Sibeko-Matjila, Kgomotso P.;Matjila, Paul T.
立克次体目的成员是小的专性细胞内细菌，其是媒介传播的，并且可以在全世界范围内引起人类的轻微至致命的疾病。关于南非农村地区狗可能携带的人畜共患立克次体病原体的信息很少。为了表征感染犬的立克次体病原体，我们在2011年10月至2012年5月期间使用反向线性印迹（RLB）和立克次体属特异性和种特异性定量实时PCR（qPCR）测定筛选了从南非普马兰加省Bushbuckridge市的家犬中收集的141份血液样本、103只蜱和43只跳蚤。RLB结果显示，49%的血液样本和30%的蜱池对埃里希体/无形体属特异性探针呈阳性; 16%的血液样本对犬埃里希体呈阳性。在36%的血液样本和30%的蜱池筛选中无法检测到血寄生虫DNA。七个（70%）蜱池和两个跳蚤池对立克次体属呈阳性;三个（30%）蜱池对非洲立克次体呈阳性;两个跳蚤池（100%）对猫立克次体呈阳性。测序证实感染了R.非洲立克次体（Rickettsia africae）和阿斯塔纳立克次体（Rickettsia asparaensis）;从其中一个R.猫科动物阳性跳蚤池。无形体属南非犬株（与嗜吞噬细胞无形体密切相关）、无形体属（A.嗜吞噬细胞菌，和恙虫病东方体样序列从血液样品中鉴定。在南非农村社区从家犬及其体外寄生虫中检测到新出现的人畜共患病病原体，突出了这些病原体可能发生的人类感染的潜在风险。
Members of the order Rickettsiales are small, obligate intracellular bacteria that are vector-borne and can cause mild to fatal diseases in humans worldwide. There is little information on the zoonotic rickettsial pathogens that may be harbored by dogs from rural localities in South Africa. To characterize rickettsial pathogens infecting dogs, we screened 141 blood samples, 103 ticks, and 43 fleas collected from domestic dogs in Bushbuckridge Municipality, Mpumalanga Province of South Africa, between October 2011 and May 2012 using the reverse line blot (RLB) and Rickettsia genus and species-specific quantitative real-time PCR (qPCR) assays. Results from RLB showed that 49% of blood samples and 30% of tick pools were positive for the genus-specific probes for Ehrlichia/Anaplasma; 16% of the blood samples were positive for Ehrlichia canis. Hemoparasite DNA could not be detected in 36% of blood samples and 30% of tick pools screened. Seven (70%) tick pools and both flea pools were positive for Rickettsia spp; three (30%) tick pools were positive for Rickettsia africae; and both flea pools (100%) were positive for Rickettsia felis. Sequencing confirmed infection with R. africae and Candidatus Rickettsia asemboensis; an R. felis-like organism from one of the R. felis-positive flea pools. Anaplasma sp. South Africa dog strain (closely related to Anaplasma phagocytophilum), A. phagocytophilum, and an Orientia tsutsugamushi-like sequence were identified from blood samples. The detection of emerging zoonotic agents from domestic dogs and their ectoparasites in a rural community in South Africa highlights the potential risk of human infection that may occur with these pathogens.