Insulin-like growth factor binding protein-1, a quick way to detect amniotic fluid
Insulin-like growth factor binding protein-1, a quick way to detect amniotic fluid
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DOI：
10.1034/j.1600-0412.1998.770307.x
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发表时间：
1998-03-01
影响因子：
4.3
通讯作者：
Lyrenas, S
中科院分区：
文献类型：
--
作者：
Darj, E;Lyrenas, S
背景胎膜早破的检测是妊娠管理的关键。各种不同的测试都有不同的局限性，用于诊断。胰岛素样生长因子结合蛋白-1(IGFBP-1)在羊水中的浓度高于血清、宫颈粘液、尿液和精浆中的浓度。一种商用试剂盒，将抗IGFBF-1的单抗固定在一根棍子上，可以买到。本研究的目的是探讨快速试纸试验能否证实或排除羊水的存在。一项涉及瑞典六个产科和妇科的多中心研究旨在评估诊断阴道内羊水存在的新试纸技术。174名女性接受了检查。46例有明显的FORM，29例无FORM，99例疑似FROM。在46名患有明显From的女性中，有44人的From测试呈阳性。29例女性中有27例阴性，其敏感性为95.7%，特异性为93.1%。可疑胎膜破裂的敏感性为70.8%，特异性为88.2%，阳性预测值(PPV)为92%。IGFBP在羊水中浓度较高。用抗IGFBP-1的单抗进行试纸试验具有快速、敏感性和特异性高的特点。它是对现有TEL库的有用补充，可以从中进行检测。
Background The detection of premature rupture of membranes (FROM) is essential to the management of pregnancy. Various tests, all with different limitations, have been used to diagnose FROM. Insulin-like growth factor binding protein-1 (IGFBP-1) is present in an essentially higher concentration in amniotic fluid, than in serum, cervical mucous, urine and seminal plasma. A commercial kit, with monoclonal antibodies to IGFBF-1 attached to a stick, is available. The aim of this study was to investigate whether a rapid dipstick test could confirm or exclude the presence of amniotic fluid.Methods. A multicenter study, involving six departments of obstetrics and gynecology in Sweden, was designed to evaluate the new dipstick technique of diagnosing the presence of amniotic fluid in the vagina. One hundred and seventy-four women were examined. Forty-six women with obvious FROM, 29 women without FROM and 99 women with suspected FROM.Results. Forty-four out of forty-six women with obvious FROM had a positive FROM-TEST. Twenty-seven out of twenty-nine women without FROM had a negative FROM-TEST, giving a sensitivity of 95.7% and a specificity of 93.1%. Among the women with suspected rupture of membranes, the sensitivity was 70.8%, the specificity 88.2% and the positive predictive value (PPV) 92%.Conclusion. IGFBP is present in high concentration in amniotic fluid. The dipstick test with monoclonal antibodies to IGFBP-1 is rapid and has a high PPS sensitivity and specificity. It is a useful complement to the existing arsenal of teals to detect FROM.