VIRUS-SPECIFIC DNA IN CYTOPLASM OF AVIAN-SARCOMA VIRUS-INFECTED CELLS IS A PRECURSOR TO COVALENTLY CLOSED CIRCULAR VIRAL-DNA IN NUCLEUS
VIRUS-SPECIFIC DNA IN CYTOPLASM OF AVIAN-SARCOMA VIRUS-INFECTED CELLS IS A PRECURSOR TO COVALENTLY CLOSED CIRCULAR VIRAL-DNA IN NUCLEUS
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DOI：
10.1128/jvi.25.1.104-104.1978
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发表时间：
1978-01-01
影响因子：
5.4
通讯作者：
VARMUS, HE
中科院分区：
文献类型：
--
作者：
SHANK, PR;VARMUS, HE
Three principal forms of viral DNA were identified in [Japanese quail fibrosarcoma QT-6] cells infected with avian sarcoma virus: a linear duplex molecule synthesized in the cytoplasm, a covalently closed circular molecule found in the nucleus and proviral DNA covalently linked to high-MW cell DNA. To define precursor-product relationships among these forms of viral DNA, pulse-chase experiments were performed using 5-bromodeoxyuridine to label by density the linear species of viral DNA in the cytoplasm during the first 4 h after infection. After a 4-8 h chase with thymidine, a portion of the density-labeled viral DNA was transported to the nucleus and converted to a covalently closed circular form. Linear viral DNA, synthesized in the cytoplasm, is apparently the precursor to closed circular DNA observed in the nucleus.
