ROLE FOR C-MYC IN ACTIVATION-INDUCED APOPTOTIC CELL-DEATH IN T-CELL HYBRIDOMAS
ROLE FOR C-MYC IN ACTIVATION-INDUCED APOPTOTIC CELL-DEATH IN T-CELL HYBRIDOMAS
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DOI：
10.1126/science.1378649
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发表时间：
1992-07-10
期刊：
影响因子：
56.9
通讯作者：
GREEN, DR
中科院分区：
文献类型：
--
作者：
SHI, YF;GLYNN, JM;GREEN, DR
当通过T细胞受体复合物激活时，未成熟T细胞和一些T细胞杂交瘤经历凋亡性细胞死亡，这一现象可能与抗原诱导的发育中T细胞的负选择有关。这种激活诱导的细胞凋亡依赖于垂死细胞中活性蛋白和RNA的合成，尽管以前没有鉴定出该过程所需的基因。对应于c-myc的反义寡核苷酸阻断T细胞杂交瘤中c-Myc蛋白的组成型表达，并干扰活化诱导的细胞凋亡的所有方面，而不影响这些细胞中的淋巴因子产生。这些数据表明，c-myc表达是激活诱导的凋亡的必要组成部分。
Immature T cells and some T cell hybridomas undergo apoptotic cell death when activated through the T cell receptor complex, a phenomenon that is probably related to antigen induced negative selection of developing T cells. This activation-induced apoptosis depends on active protein and RNA synthesis in the dying cells, although none of the genes required for this process have previously been identified. Antisense oligonucleotides corresponding to c-myc block the constitutive expression of c-Myc protein in T cell hybridomas and interfere with all aspects of activation-induced apoptosis without affecting lymphokine production in these cells. These data indicate that c-myc expression is a necessary component of activation-induced apoptosis.