Amadori-glycated phosphatidylethanolamine induces angiogenic differentiations in cultured human umbilical vein endothelial cells
Amadori-glycated phosphatidylethanolamine induces angiogenic differentiations in cultured human umbilical vein endothelial cells
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DOI：
10.1016/s0014-5793(03)01237-7
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发表时间：
2003-12-04
期刊：
影响因子：
3.5
通讯作者：
Miyazawa, T
中科院分区：
文献类型：
--
作者：
Oak, JH;Nakagawa, K;Miyazawa, T
糖基化与内皮功能障碍有关，内皮功能障碍导致糖尿病和衰老相关的血管并发症。本研究的目的是确定amadori -糖化磷脂酰乙醇胺（Amadori-PE）是否影响培养的人脐静脉内皮细胞（HUVEC）的增殖、迁移和管的形成。Amadori-PE是一种脂质糖基化化合物，在高血糖状态下形成的速率增加。低于5mum的低浓度Amadori-PE显著增强了这三个参与血管生成的因子。此外，Amadori-PE刺激HUVEC导致基质金属蛋白酶2 （MMP-2）的分泌，这是血管生成初始阶段的关键酶。我们的研究结果首次证明Amadori-PE可能是一种重要的化合物，由于其对内皮细胞的血管生成活性而促进血管疾病。我们还证明MMP-2是amadori - pe驱动的血管生成的主要介质。(C) 2003年欧洲生化学会联合会。Elsevier B.V.版权所有。
Glycation has been implicated in the endothelial dysfunction that contributes to both diabetes- and aging-associated vascular complications. The aim of the present study was to determine whether Amadori-glycated phosphatidylethanolamine (Amadori-PE), a lipid-linked glycation compound that is formed at an increased rate in hyperglycemic states, affected proliferation, migration and tube formation of cultured human umbilical vein endothelial cells (HUVEC). Amadori-PE at a low concentration of less than 5 muM significantly enhanced these three factors involved in angiogenesis. Furthermore, stimulation of HUVEC with Amadori-PE resulted in secretion of matrix metalloproteinase 2 (MMP-2), a pivotal enzyme in the initial step of angiogenesis. Our results demonstrated for the first time that Amadori-PE may be an important compound that promotes vascular disease as a result of its angiogenic activity on endothelial cells. We also demonstrated that MMP-2 is a primary mediator of Amadori-PE-driven angiogenesis. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.