The interaction of gamma-crystallins with model surfaces.
The interaction of gamma-crystallins with model surfaces.
复制标题
γ-晶状体蛋白与模型表面的相互作用。
DOI：
10.1016/0003-9861(91)90145-9
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发表时间：
1991
影响因子：
3.9
通讯作者：
Middaugh,CR
中科院分区：
文献类型：
--
作者：
Matsuno,K;Lewis,RV;Middaugh,CR
Three biophysical techniques were employed to study the structure and thermal stability of a series of homologous bovine lens γ-crystallins upon binding to three model surfaces. The surfaces in order of increasing hydrophobicity were silica, methyl silica, and diphenyl silica. Secondary structure was analyzed by deconvolution Fourier transform infrared spectroscopy, while tertiary structure alterations were probed by front surface fluorescence spectroscopy. The effect of surface binding on protein thermal stability was analyzed by fluorescence and differential scanning calorimetry. The comparison of free and surface-bound protein with variations in the electrostatic and hydrophobic character of both the protein and the adsorbent surface with these techniques demonstrated that: (i) destabilization on hydrophobic surfaces is greater than on a more hydrophilic interface, (ii) detectable conformational changes tend to increase as the hydrophobicity of the surface increases, and (iii) subtle structural differences among proteins can play an important role in determining differences in protein stability and structure upon surface adsorption.
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AIMS 健康状况测量方法：概念背景和测量属性。
DOI：
--
发表时间：
1982
期刊：
The Journal of rheumatology
影响因子：
--
作者：
Meenan,RF
通讯作者：
Meenan,RF
影响因子：
22.4
作者：
GUADAGNOLI, E;VELICER, WF
通讯作者：
VELICER, WF
健康结果的维度：健康评估问卷、残疾和疼痛量表。
影响因子：
3.9
作者：
J. Fries;P. Spitz;D. Young
通讯作者：
D. Young
随机临床试验中多重比较的影响。
影响因子：
5.9
作者：
David H.G. Smith;David H.G. Smith;John D. Clemens;William B. Crede;Mary R. Harvey;E. J. Gracely;E. J. Gracely
通讯作者：
E. J. Gracely
低剂量 D-青霉胺治疗类风湿性关节炎。
影响因子：
--
作者：
H. Williams;J. Ward;J. Reading;M. Egger;J. T. Grandone;J. T. Grandone;C. Samuelson;D. Furst;D. Furst;J. M. Sullivan;J. M. Sullivan;M. Watson;M. Watson;M. Guttadauria;M. Guttadauria;E. Cathcart;E. Cathcart;S. Kaplan;S. Kaplan;J. Halla;J. Halla;A. Weinstein;A. Weinstein;P. Plotz
通讯作者：
P. Plotz