Non-invasive visualization of epidermal responses to injury using a fluorescent transgenic reporter
Non-invasive visualization of epidermal responses to injury using a fluorescent transgenic reporter
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DOI：
10.1111/j.0022-202x.2004.23486.x
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发表时间：
2004-11-01
影响因子：
6.5
通讯作者：
Sanes, JR
中科院分区：
文献类型：
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作者：
Pan, YA;Sanes, JR
我们描述了转基因小鼠，其中表达的报告，黄色荧光蛋白（YFP），是局部激活的表皮细胞损伤部位。YFP在受伤后一天就可以检测到;其水平在3天内达到峰值，然后在随后的一周内下降。表达也被化学刺激物激活，并被局部施用氢化可的松抑制。这些小鼠允许非侵入性的延时监测对体内损伤的反应。它们可用于检测表皮激活和测试可能引起或减弱表皮反应的试剂。
We describe transgenic mice in which expression of a reporter, the yellow fluorescent protein (YFP), is locally activated in epidermal cells at sites of injury. YFP is detectable a day after injury; its levels peak within 3 d, and then decline over the subsequent week. Expression is also activated by a chemical irritant, and is suppressed by topical administration of hydrocortisone. These mice permit non-invasive time-lapse monitoring of responses to injury in vivo. They can be used to detect epidermal activation and to test agents that may provoke or attenuate epidermal responses.