PROTON SECRETION BY STIMULATED NEUTROPHILS - SIGNIFICANCE OF HEXOSE-MONOPHOSPHATE SHUNT ACTIVITY AS SOURCE OF ELECTRONS AND PROTONS FOR THE RESPIRATORY BURST
PROTON SECRETION BY STIMULATED NEUTROPHILS - SIGNIFICANCE OF HEXOSE-MONOPHOSPHATE SHUNT ACTIVITY AS SOURCE OF ELECTRONS AND PROTONS FOR THE RESPIRATORY BURST
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DOI：
10.1172/jci111442
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发表时间：
1984-01-01
影响因子：
15.9
通讯作者：
TAUBER, AI
中科院分区：
文献类型：
--
作者：
BORREGAARD, N;SCHWARTZ, JH;TAUBER, AI
[人类]中性粒细胞的吞噬作用伴随着氧气消耗的爆发和磷酸己糖分流（HMPS）的激活。与消耗的O2量相等的质子分泌是呼吸爆发的另一个特征，但其来源未被确定，呼吸爆发中捐赠给O2的所有电子的来源也未被确定。在人类嗜中性粒细胞中的总CO2生成进行了定量和质子分泌引起的吞噬作用被认为是伴随着化学计量增加CO2生成。添加碳酸酐酶及其抑制剂对测量的CO2量或分泌的质子量没有影响。在刺激的中性粒细胞的呼吸爆发中产生的CO2水合形成H2CO3，然后解离，解释了观察到的质子分泌。产生的CO2相当于消耗的O2，呼吸商接近1。HMPS活性是NADPH氧化酶的电子和与呼吸爆发相关的质子分泌的来源。
Phagocytosis by [human] neutrophils is accompanied by a burst in O2 consumption and activation of the hexose monophosphate shunt (HMPS). Proton secretion equal to the amount of O2 consumed is an additional feature of the respiratory burst, but its source was not identified, nor was the source of all electrons donated to O2 in the respiratory burst. Total CO2 generation in human neutrophils was quantitated and proton secretion elicited by phagocytosis was found to be accompanied by a stoichiometric increase in CO2 generation. Addition of carbonic anhydrase and its inhibitors had no effect on either the quantities of CO2 measured or the quantities of protons secreted. The CO2 generated in the respiratory burst of stimulated neutrophils is hydrated to form H2CO3, which then dissociates, accounting for the observed proton secretion. The CO2 generated corresponds to the O2 consumed with a respiratory quotient of nearly 1. The HMPS activity is the source of both the electrons for the NADPH oxidase and of protons secreted in association with the respiratory burst.