Vascular Structure and Function in the Medial Collateral Ligament of Anterior Cruciate Ligament Transected Rabbit Knees
Vascular Structure and Function in the Medial Collateral Ligament of Anterior Cruciate Ligament Transected Rabbit Knees
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DOI：
10.1002/jor.22643
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发表时间：
2014-09-01
影响因子：
2.8
通讯作者：
Bray, Robert C.
中科院分区：
文献类型：
--
作者：
Miller, Daniel;DeSutter, Christopher;Bray, Robert C.
确定前交叉韧带横断（ACL-t）兔膝内侧副韧带（MCL）血管反应性降低是否是由于与血管生成相关的周细胞缺乏所致。采用激光散斑灌注成像技术，在ACL-t兔膝关节（n = 6）和对照膝关节（n = 5）中评价氯化钾（KCl）、苯丙氨酸、乙酰胆碱和硝普钠（SNP）的血管反应。通过超声成像确定韧带变性。血管和周细胞体积的测量使用定量免疫组织化学容量分析，使用CD 31和α-平滑肌肌动蛋白抗体与共定位分析。ACL-t兔的灌注增加了2.5倍。ACL膝关节对苯肾上腺素、SNP和乙酰胆碱的反应性显著降低，而KCl反应没有变化。MCL超声成像显示ACL-t MCL中胶原组织减少，韧带厚度增加，含水量增加。ACL缺陷膝关节的血管体积增加了四倍，而周细胞体积与内皮细胞体积之比没有变化。CD 31和α-SMA共定位无差异。ACL-t膝关节MCL中的血管不缺乏平滑肌。MCL脉管系统可经历对KCl的收缩反应，但具有受损的受体介导的反应和受损的一氧化氮信号传导。(C)2014骨科研究学会。由威利期刊公司出版
To determine if decreased vascular responsiveness in the medial collateral ligament (MCL) of anterior cruciate ligament transected (ACL-t) rabbit knees is due to pericyte deficiency associated with angiogenesis. Vascular responses to potassium chloride (KCl), phenylephrine, acetylcholine, and sodium nitroprusside (SNP) were evaluated in ACL-t rabbit knees (n = 6) and control knees (n = 5) using laser speckle perfusion imaging. Ligament degeneration was determined by ultrasound imaging. Vascular and pericyte volume were measured using quantitative immunohistochemical volumetric analysis using CD31 and alpha-smooth muscle actin antibodies with co-localization analysis. Perfusion was increased in the ACL-t rabbits 2.5-fold. Responsiveness to phenylephrine, SNP, and acetylcholine was significantly decreased in the ACL knee while no change in KCl responses was seen. MCL ultrasound imaging revealed decreased collagen organization, increased ligament thickness, and increased water content in the ACL-t MCL. Vascular Volume was increased fourfold in ACL deficient knees, while pericyte volume to endothelial volume was not changed. No difference in CD31 and alpha-SMA co-localization was found. Blood vessels in the MCL of ACL-t knees do not lack smooth muscle. The MCL vasculature can undergo constrictive response to KCl, but have impaired receptor mediated responses and impaired nitric oxide signaling. (C) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.