A radiation hybrid map of chicken Chromosome 4
A radiation hybrid map of chicken Chromosome 4
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DOI：
10.1007/s00335-004-2362-8
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发表时间：
2004-07-01
期刊：
影响因子：
2.5
通讯作者：
Groenen, MAM
中科院分区：
文献类型：
--
作者：
Rabie, TSKM;Crooijmans, RPMA;Groenen, MAM
通过辐射杂交（RH）作图和细菌人工染色体（BAC）作图相结合，提高了鸡4号染色体（GGA4）物理图谱的作图分辨率。 ChickRH6 混合组用于构建 GGA4 的 RH 图谱。已知位于 GGA4 上的 11 个微卫星被作为该染色体遗传连锁图的锚点。基于 GGA4 与人类 4 号和 X 号染色体之间已知的保守同线性，通过 BLAST 分析从这些人类染色体中鉴定出直系同源鸡基因的序列。这些序列随后用于开发要在 RH 面板上分型的 STS 标记。使用对数对数 (LOD) 阈值 5.0，可以构建九个连锁群，这些连锁群与该染色体的遗传连锁图比对。生成的 RH 图谱由 11 个微卫星标记和 50 个基因组成。为了进一步增加图谱上的基因数量并为该染色体的物理 BAC 图谱提供额外的锚点，鉴定了 22 个微卫星和 99 个基因的 BAC 克隆。结合 RH 和 BAC 作图方法，在 GGA4 上绘制了 61 个基因，提高了该染色体鸡-人比较图谱的分辨率。这种增强的比较图谱分辨率能够识别 GGA4 与人类染色体 4q 和 Xp 之间的多重重排。
The mapping resolution of the physical map for chicken Chromosome 4 (GGA4) was improved by a combination of radiation hybrid (RH) mapping and bacterial artificial chromosome (BAC) mapping. The ChickRH6 hybrid panel was used to construct an RH map of GGA4. Eleven microsatellites known to be located on GGA4 were included as anchors to the genetic linkage map for this chromosome. Based on the known conserved synteny between GGA4 and human Chromosomes 4 and X, sequences were identified for the orthologous chicken genes from these human chromosomes by BLAST analysis. These sequences were subsequently used for the development of STS markers to be typed on the RH panel. Using a logarithm of the odds (LOD) threshold of 5.0, nine linkage groups could be constructed which were aligned with the genetic linkage map of this chromosome. The resulting RH map consisted of the 11 microsatellite markers and 50 genes. To further increase the number of genes on the map and to provide additional anchor points for the physical BAC map of this chromosome, BAC clones were identified for 22 microsatellites and 99 genes. The combined RH and BAC mapping approach resulted in the mapping of 61 genes on GGA4 increasing the resolution of the chicken-human comparative map for this chromosome. This enhanced comparative mapping resolution enabled the identification of multiple rearrangements between GGA4 and human Chromosomes 4q and Xp.