THE FULL-LENGTH NUCLEOTIDE-SEQUENCES OF THE VIRULENT TRINIDAD DONKEY STRAIN OF VENEZUELAN EQUINE ENCEPHALITIS-VIRUS AND ITS ATTENUATED VACCINE DERIVATIVE, STRAIN TC-83
THE FULL-LENGTH NUCLEOTIDE-SEQUENCES OF THE VIRULENT TRINIDAD DONKEY STRAIN OF VENEZUELAN EQUINE ENCEPHALITIS-VIRUS AND ITS ATTENUATED VACCINE DERIVATIVE, STRAIN TC-83
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DOI：
10.1016/0042-6822(89)90347-4
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发表时间：
1989-05-01
期刊：
影响因子：
3.7
通讯作者：
TRENT, DW
中科院分区：
文献类型：
--
作者：
KINNEY, RM;JOHNSON, BJB;TRENT, DW
对特立尼达驴（TRD）委内瑞拉马脑炎（VEE）病毒及其衍生疫苗TC-83的全基因组cDNA克隆的核苷酸序列分析，揭示了TC-83病毒与其亲本基因组之间的11个差异。TC-83基因组的5' -非编码区和3' -非编码区分别发生1个核苷酸替换和1个核苷酸缺失。两种病毒非结构多肽的氨基酸序列仅在非结构蛋白（nsP） 3的260个氨基酸位置上有保守的Ser （TRD）到Thr （TC-83）取代。TC-83病毒的2个沉默突变（E1和E2各1个），E1糖蛋白1个氨基酸替换，E2包膜糖蛋白5个氨基酸替换（b.j.b. Johnson， R. M. Kinney， C. L. Kost, and D. W. Trent, 1986， J. Gen. Virol. 67, 1951-1960）。TRD病毒基因组长11444个核苷酸，其中5”的非编码区有44个核苷酸。VEE nsP3的羧基末端含有两个肽段（长7和34个氨基酸），具有高保真度。非结构多蛋白的开放阅读框被nsP3和nsP4之间的框架内蛋白石终止密码子中断，正如Sindbis， Ross River和Middelburg病毒所报道的那样。推测得到的VEE TRD nsP1、nsP2、nsP3和nsP4多肽与Sindbis和Semliki Forest病毒同源多肽比对序列的同源性分别为60-66%、57-58%、35-44%和73-71%。病毒的nsP3缺乏同源性是由于该多肽羧基末端一半的序列变化。
Nucletoide sequence analysis of cDNA clones covering the entire genomes of Trinidad donkey (TRD) Venezuelan equine encephalitis (VEE) virus and its vaccine derivative, TC-83, has revealed 11 differences between the genomes of TC-83 virus and its parent. One nucleotide substitution and a single nucleotide deletion occurred in the 5''- and 3''-noncoding regions of the TC-83 genome, respectively. The deduced amino acid sequences of the nonstructural polypeptides of the two viruses differed only in a conservative Ser (TRD) to Thr (TC-83) substitution in nonstructural protein (nsP) three at amino acid position 260. The two silent mutations (one each in E1 and E2), one amino acid substitution in the E1 glycoprotein, and five substitutions in the E2 envelope glycoprotein of TC-83 virus were reported previously (B. J. B. Johnson, R. M. Kinney, C. L. Kost, and D. W. Trent, 1986, J. Gen. Virol. 67, 1951-1960). The genome of TRD virus was 11,444 nucleotides long with a 5''-noncoding region of 44 nucleotides. The carboxyl terminal portion of VEE nsP3 contained two peptide segments (7 and 34 amino acids long) that were repeated with high fidelity. The open reading frame of the nonstructural polyprotein was interrupted by an in-frame opal termination codon between nsP3 and nsP4, as has been reported for Sindbis, Ross River, and Middelburg viruses. The deduced amino acid sequences of the VEE TRD nsP1, nsP2 nsP3, and nsP4 polypeptides showed 60-66%, 57-58%, 35-44%, and 73-71% identity with the aligned sequences of the cognate polypeptides of Sindbis and Semliki Forest viruses, respectively. The lack of homology in the nsP3 of the viruses is due to sequence variation in the carboxyl terminal half of this polypeptide.