Deciphering the iron response in Acinetobacter baumannii: A proteomics approach.
Deciphering the iron response in Acinetobacter baumannii: A proteomics approach.
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DOI：
10.1016/j.jprot.2010.07.010
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Actis LA
中科院分区：
文献类型：
--
作者：
Nwugo CC;Gaddy JA;Zimbler DL;Actis LA
铁是一种重要的营养物质，在细菌差异基因表达和蛋白质生产中起着重要作用。因此，采用二维凝胶电泳-质谱法对富铁和螯合条件下培养的鲍曼不动杆菌ATCC 19606T细胞的总裂解液和外膜组分进行比较分析，鉴定出58个差异产生的蛋白点。其中19个和35个分别代表铁抑制和铁诱导的蛋白质点，另外4个代表与铁无关的金属螯合反应。大多数铁抑制蛋白点代表外膜铁载体受体，其中一些可能参与利用其他细菌产生的铁载体。铁诱导的蛋白点代表了广泛的蛋白质，包括参与铁储存的蛋白质，如Bfr，代谢和能量过程的蛋白质，如AcnA， AcnB, GlyA， SdhA和SodB，以及脂质生物合成。铁调控的Hfq同源物的检测表明，该细菌中的铁调控可能像其他细菌中描述的那样由毛皮和小rna介导。铁诱导的OmpA的产生表明该蛋白在铁代谢中起作用，正如在铁螯合条件下OmpA等基因缺陷衍生物的生长能力减弱所表明的那样。
Iron is an essential nutrient that plays a role in bacterial differential gene expression and protein production. Accordingly, the comparative analysis of total lysate and outer membrane fractions isolated from A. baumannii ATCC 19606T cells cultured under iron-rich and -chelated conditions using 2-D gel electrophoresis-mass spectrometry resulted in the identification of 58 protein spots differentially produced. While 19 and 35 of them represent iron-repressed and iron-induced protein spots, respectively, four other spots represent a metal chelation response unrelated to iron. Most of the iron-repressed protein spots represent outer membrane siderophore receptors, some of which could be involved in the utilization of siderophores produced by other bacteria. The iron-induced protein spots represent a wide range of proteins including those involved in iron storage, such as Bfr, metabolic and energy processes, such as AcnA, AcnB, GlyA, SdhA, and SodB, as well as lipid biosynthesis. The detection of an iron-regulated Hfq ortholog indicates that iron regulation in this bacterium could be mediated by Fur and small RNAs as described in other bacteria. The iron-induced production of OmpA suggests this protein plays a role in iron metabolism as shown by the diminished ability of an OmpA isogenic deficient derivative to grow under iron-chelated conditions.
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影响因子：
3.2
作者：
Ge, Li;Seah, Stephen Y. K.
通讯作者：
Seah, Stephen Y. K.
影响因子：
2.8
作者：
Dorsey, CW;Tolmasky, ME;Actis, LA
通讯作者：
Actis, LA
影响因子：
2.8
作者：
Dorsey, CW;Tomaras, AP;Actis, LA
通讯作者：
Actis, LA
影响因子：
3.2
作者：
ANDREWS, SC;HARRISON, PM;GUEST, JR
通讯作者：
GUEST, JR
DOI：
10.1007/bf00269672
发表时间：
1981-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HANTKE, K
通讯作者：
HANTKE, K