The effect of intrauterine infusion of inflammation-provoking factors on proinflammatory cytokines and hormones in rat peripheral blood.
The effect of intrauterine infusion of inflammation-provoking factors on proinflammatory cytokines and hormones in rat peripheral blood.
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发表时间：
2005
影响因子：
0.8
通讯作者：
B. Jana;J. Kucharski;M. Jedlińska-Krakowska;E. Wolińska‐Witort
中科院分区：
文献类型：
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作者：
B. Jana;J. Kucharski;M. Jedlińska-Krakowska;E. Wolińska‐Witort
这项研究的目的是利用大鼠模型确定子宫感染是否会导致外周血中细胞因子浓度的增加，以及这种增加是否伴随着垂体-卵巢轴功能的变化。于动情后期宫内注射脂多糖(15微克)、肽聚糖(1 Mg)、大肠杆菌(10(6)CFU)悬液，测定血浆肿瘤坏死因子-α、白介素1β、黄体生成素、卵泡刺激素、催乳素、孕酮、睾酮、雌二醇-17β水平及子宫重量。分别于治疗后第3、7、10天处死大鼠，采集血样。治疗后所有大鼠均出现子宫炎症和阴道分泌物。注射脂多糖、肽聚糖和大肠杆菌可引起卵巢周期活动的显著变化，主要表现为发情间期。所有这些因素的应用均导致大鼠血浆肿瘤坏死因子-α、白介素1β水平升高(P<；0.05，P<；0.01)，主要发生在第3天和第7天。病理因素组大鼠血浆黄体生成素、卵泡刺激素、催乳素和雌二醇-17β水平降低(P<；0.05，P<；0.01)，孕酮和睾酮水平升高(P<；0.05)。这些结果表明，大鼠输注脂多糖、肽聚糖和大肠杆菌后子宫的炎症发展过程与外周血中肿瘤坏死因子-α、白介素1β浓度的升高有关，并伴随着垂体轴-卵巢轴功能的改变。
The aim of the study was to determine, using the rat model, whether uterine infections cause an increase in cytokine concentrations in peripheral blood, and whether this increase is accompanied by changes in the pituitary-ovarian axis function. The levels of tumor necrosis factor-alpha, interleukin-1beta, luteinizing hormone, follicle-stimulating hormone, prolactin, progesterone, testosterone and estradiol-17beta in blood plasma as well as the weight of the uterus were determined after intrauterine infusion of lipopolysaccharide (15 microg), peptidoglycan (1 mg) and Escherichia coli (10(6) cfu) suspension on the day of metaestrus. On days 3, 7 and 10 after treatment the rats were sacrificed to collect the blood samples. Inflammation of uterus and vaginal discharge developed in all rats after treatment. The administration of lipopolysaccharide, peptidoglycan and Escherichia coli induced considerable changes in ovarian cyclic activity, mainly diestrus was observed. Application of all these factors resulted in an increase (P<0.05, P<0.01) of plasma levels of tumor necrosis factor-alpha, interleukin-1beta, mainly on day 3 and 7. In the rats receiving pathological factors, the plasma levels of luteinizing hormone, follicle-stimulating hormone, prolactin and estradiol-17beta decreased (P<0.05, P<0.01) whereas progesterone and testosterone increased (P<0.05). These results indicate that in rats, the developing inflammatory process of the uterus following lipopolysaccharide, peptidoglycan and Escherichia coli infusions is connected with an increase of tumor necrosis factor-alpha, interleukin-1beta concentrations in peripheral blood, and is accompanied by changes in the pituitary-ovarian axis function.