Purification and properties of L-methionine γ-lyase from Aeromonas sp.
Purification and properties of L-methionine γ-lyase from Aeromonas sp.
复制标题
气单胞菌 L-蛋氨酸 γ-裂解酶的纯化和性质。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
K. Soda
中科院分区：
文献类型：
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作者：
Torn Nakayama;N. Esaki;Woon Joo Lee;I. Tanaka;Hidehiko Tanaka;K. Soda
甲硫醇和氨。我们从卵圆假单胞菌（Pseudomonasputida, = Ps. ovalis）中纯化了该酶并对其进行了表征。2)基于该酶的多重催化功能，如前所述，它适用于生产各种光学活性的硫和硒氨基酸。这种酶的有用性表明了获得一种能有效生产这种酶的微生物的必要性。我们从湖水中分离出一株l -蛋氨酸y-裂解酶活性（每细胞湿重）比恶臭单胞菌高10倍的菌株，并将其鉴定为气单胞菌。其中描述了从细菌细胞中纯化酶到均匀性及其特性。我们已经做了许多尝试，分离出可以在含有0.5% l -蛋氨酸作为唯一碳和氮源的培养基（pH 7.2）中生长的微生物。从土壤、排水、河流和湖泊中分离出的几种细菌具有高的l -蛋氨酸y-裂解酶
methanethiol and ammonia. We have purified the enzyme from Pseudomonasputida ( = Ps. ovalis) to characterize it. 1 >2) Based on the multi-catalytic function of the enzyme it is applicable to the production of various optically active sulfur and selenium amino acids as reviewed previously.3) This usefulness of the enzymehas shownthe necessity of obtaining an microorganism that produces the enzyme effectively. We have isolated bacterial strain from lake water that shows 10-times higher L-methionine y-lyase activity (per wet weight of cells) than Ps. putida, and identified it as Aeromonas sp. in the present study. Wehere describe the purification of the enzyme from the bacterial cells to homogeneity and its characterization. We have made many attempts to isolate microorganisms that can grow in a medium (pH 7.2) containing 0.5% L-methionine as a sole carbon and nitrogen source. Several bacterial strains isolated from soil, and drainage, river and lake water showed high L-methionine y-lyase