BIOSYNTHESIS OF AROMATIC-COMPOUNDS - C-13 NMR-SPECTROSCOPY OF WHOLE ESCHERICHIA-COLI-CELLS
BIOSYNTHESIS OF AROMATIC-COMPOUNDS - C-13 NMR-SPECTROSCOPY OF WHOLE ESCHERICHIA-COLI-CELLS
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DOI：
10.1073/pnas.79.19.5828
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
HERRMANN, KM
中科院分区：
文献类型：
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作者：
OGINO, T;GARNER, C;HERRMANN, KM
野生型E.大肠杆菌显示共振来自糖酵解和ATP形成的代谢中间体，但没有检测到来自芳香族氨基酸的信号。在含有aroF的反馈抗性等位基因的细胞中观察到从D-[1- 13 C]葡萄糖的酪氨酸生物合成，aroF是编码酪氨酸敏感性3-脱氧-D-阿拉伯-二-庚酮糖酸-7-磷酸合酶[7-磷酸-2-酮-3-脱氧-D-阿拉伯-庚糖酸D-赤藓糖-4-磷酸-裂解酶（赤藓糖酸-磷酸化），EC 4.1.2.15]的基因，其是控制C流经常见芳香族生物合成途径的同工酶之一。在携带多拷贝质粒的细胞中观察到酪氨酸和苯丙氨酸的类似积累，所述多拷贝质粒除了pheA和tyrA（用于控制苯丙氨酸和酪氨酸生物合成的末端途径的酶的结构基因）之外还携带野生型aroF等位基因。这些在体内测量的非侵入性探针建议反馈抑制作为定量的主要机制控制C流在共同的芳香族化合物的生物合成途径。在积累芳香族氨基酸的菌株中，检测到海藻糖的瞬时积累，表明以前未知的E。大肠杆菌的代谢伴随着芳香化合物的过量产生。
13C and 31P NMR spectra of wild-type E. coli showed resonances from metabolic intermediates of glycolysis and ATP formation, but no detectable signals from aromatic amino acids. Tyrosine biosynthesis from D-[1-13C]glucose was observed in cells harboring a feedback-resistant allele of aroF, the gene encoding tyrosine-sensitive 3-deoxy-D-ara-bino-heptulosonate-7-phosphate synthase [7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), EC 4.1.2.15], one of the isoenzymes that control C flow through the common aromatic biosynthetic pathway. A similar accumulation of tyrosine and phenylalanine is seen in cells carrying a multiple-copy plasmid that carries a wild-type aroF allele in addition to pheA and tyrA, the structural genes for controlling enzymes of the terminal pathways to phenylalanine and tyrosine biosynthesis. These in vivo measurements by a noninvasive probe suggest feedback inhibition as the quantitatively major mechanism controlling C flow in the common aromatic compound biosynthetic pathway. In strains accumulating aromatic amino acids, a transient accumulation of trehalose was detected, indicating that previously unknown changes in E. coli metabolism accompany overproduction of aromatic compounds.