Electron Tomography Reveals Polyhedrin Binding and Existence of both Empty and Full Cytoplasmic Polyhedrosis Virus Particles inside Infectious Polyhedra
Electron Tomography Reveals Polyhedrin Binding and Existence of both Empty and Full Cytoplasmic Polyhedrosis Virus Particles inside Infectious Polyhedra
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电子断层扫描揭示多面体结合以及感染性多面体内部空和全胞质多角体病毒颗粒的存在
DOI：
10.1128/jvi.00103-11
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发表时间：
2011-06-01
影响因子：
5.4
通讯作者：
Zhou, Z. Hong
中科院分区：
文献类型：
--
作者：
Chen, Justin;Sun, Jingchen;Zhou, Z. Hong
摘要 先前的研究已经描述了纯化的胞质多角体病毒（CPV）和多角体蛋白的结构。然而，多面体分子如何将 CPV 颗粒嵌入感染性多面体中尚不清楚。通过使用电子断层扫描，我们表明 CPV 颗粒以随机空间分布被封闭在多面体晶格内，并通过 A 尖峰而不是之前认为的通过 B 尖峰与多角体蛋白相互作用。此外，在多面体内部发现了完整的（有RNA）和空的（无RNA）衣壳，这表明体内CPV组装存在自发的RNA衣壳化过程。
ABSTRACT Previous studies have described the structure of purified cytoplasmic polyhedrosis virus (CPV) and that of polyhedrin protein. However, how polyhedrin molecules embed CPV particles inside infectious polyhedra is not known. By using electron tomography, we show that CPV particles are occluded within the polyhedrin crystalline lattice with a random spatial distribution and interact with the polyhedrin protein through the A-spike rather than as previously thought through the B-spike. Furthermore, both full (with RNA) and empty (no RNA) capsids were found inside polyhedra, suggesting a spontaneous RNA encapsidating process for CPV assembly in vivo.