Codon usage in the Mycobacterium tuberculosis complex
Codon usage in the Mycobacterium tuberculosis complex
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DOI：
10.1099/00221287-142-4-915
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发表时间：
1996-04-01
期刊：
影响因子：
--
通讯作者：
Sharp, PM
中科院分区：
文献类型：
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作者：
Andersson, SGE;Sharp, PM
对结核分枝杆菌(和牛分枝杆菌)基因中替代同义密码子的使用进行了研究。该物种是高Ci+C革兰氏阳性细菌的一员，基因组G+C含量约为65mol%，这种G+C含量反映在每个氨基酸对C-和G-结尾密码子的强烈偏爱：总体而言，密码子第三位的G+C含量为83%。然而，密码子的使用模式在基因之间存在显著差异，这似乎与基因表达水平有关。从基因间的变异中，确定了15个氨基酸的最佳密码子。结核分枝杆菌基因对最佳密码子的偏爱程度与来自大肠杆菌和枯草芽孢杆菌的同源物中的密码子偏爱程度有关，在结核分枝杆菌和另一种高G+C革兰氏阳性菌-谷氨酸棒杆菌之间，选择性偏爱的密码子集似乎相当保守，尽管后者的基因组和总体密码子使用量远低于G+C丰富。
The usage of alternative synonymous codons in Mycobacterium tuberculosis (and M. bovis) genes has been investigated. This species is a member of the high-Ci + C Gram-positive bacteria, with a genomic G + C content around 65 mol%, This G + C-richness is reflected in a strong bias towards C- and G-ending codons for every amino acid: overall, the G + C content at the third positions of codons is 83 %. However, there is significant variation in codon usage patterns among genes, which appears to be associated with gene expression level. From the variation among genes, putative optimal codons were identified for 15 amino acids. The degree of bias towards optimal codons in an M. tuberculosis gene is correlated with that in homologues from Escherichia coli and Bacillus subtilis, The set of selectively favoured codons seems to be quite highly conserved between M. tuberculosis and another high-G + C Gram-positive bacterium, Corynebacterium glutamicum, even though the genome and overall codon usage of the latter are much less G + C-rich.