Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events.-猫眼课题宝

DOI：

10.1016/j.devcel.2022.08.002

发表时间：

2022-08

期刊：

Developmental cell

影响因子：

11.8

通讯作者：

中科院分区：

生物学1区

文献类型：

作者：

Mario A. Mendieta-Serrano;Sunandan Dhar;Boon Heng Ng;R. Narayanan;Jorge J.Y. Lee;H. T. Ong;P. Toh;A. Röllin;Sudipto Roy;T. Saunders

骨骼肌发生是动态的，它涉及细胞形状的变化以及细胞融合和重排。然而，最终的肌肉排列是高度组织化的横纹纤维。通过结合实时成像和定量分析，我们解剖了斑马鱼肌节内的快速收缩肌细胞融合。我们发现了一个强大的内外偏置融合时间，但是，在细胞规模，有异质性的细胞形状和快速肌细胞的初始位置之间的关系，并产生融合的合作伙伴。我们表明，表达的fusogenmyomakeris允许的，但没有指导意义，在确定的时空融合模式。相反，我们观察到缓慢肌肉重排和快速肌细胞融合之间的密切协调。在缺乏慢纤维的突变体中，时空融合模式实质上更嘈杂。我们提出了一个模型，在这个模型中，缓慢的肌肉通过使它们靠近在一起，增加融合的可能性来引导肌细胞。因此，尽管融合是高度随机的，但在组织尺度上出现了稳健的肌节结构。

Skeletal myogenesis is dynamic, and it involves cell-shape changes together with cell fusion and rearrangements. However, the final muscle arrangement is highly organized with striated fibers. By combining live imaging with quantitative analyses, we dissected fast-twitch myocyte fusion within the zebrafish myotomein toto. We found a strong mediolateral bias in fusion timing; however, at a cellular scale, there was heterogeneity in cell shape and the relationship between initial position of fast myocytes and resulting fusion partners. We show that the expression of the fusogenmyomakeris permissive, but not instructive, in determining the spatiotemporal fusion pattern. Rather, we observed a close coordination between slow muscle rearrangements and fast myocyte fusion. In mutants that lack slow fibers, the spatiotemporal fusion pattern is substantially noisier. We propose a model in which slow musclesguidefast myocytes by funneling them close together, enhancing fusion probability. Thus, despite fusion being highly stochastic, a robust myotome structure emerges at the tissue scale.

Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events.

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