Covalent conjugation of bioactive peptides to graphene oxide for biomedical applications
Covalent conjugation of bioactive peptides to graphene oxide for biomedical applications
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DOI：
10.1039/c9bm00867e
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发表时间：
2019-09-01
影响因子：
6.6
通讯作者：
Sydlik, Stefanie A.
中科院分区：
文献类型：
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作者：
Eckhart, Karoline E.;Holt, Brian D.;Sydlik, Stefanie A.
石墨烯因其机械完整性、长程有序性和导电性而成为生物医学植入应用中的一种有价值的材料；但石墨烯必须经过化学修饰才能提高生物相容性并最大限度地发挥体内功能。在这里，我们开发了一种用生物活性分子共价功能化还原 GO 的基础合成方法，重点关注已显示成骨或神经生成能力的合成肽作为典型例子。 X 射线光电子能谱提供了肽与石墨烯主链共价连接的证据。这些肽-石墨烯 (Pep-G) 缀合物材料可以加工成机械坚固的三维结构。静电荷的差异使得 Pep-G 缀合物能够形成自组装的逐层涂层。此外，Pep-G 缀合物具有细胞相容性和导电性，这使我们能够研究它们作为再生支架的潜力，因为导电表面可以刺激骨骼和神经再生。值得注意的是，与对照组相比，在电刺激 Pep-G 支架上生长的 PC12 细胞表现出增强的粘附力和神经突生长。这里开发的功能化策略可用于将多种生物活性分子与氧化石墨烯缀合，以创建生物医学支架材料的细胞指导表面。
Graphene is a valuable material in biomedical implant applications due to its mechanical integrity, long-range order, and conductivity; but graphene must be chemically modified to increase biocompatibility and maximize functionality in the body. Here, we developed a foundational synthetic method for covalently functionalizing a reduced GO with bioactive molecules, focusing on synthetic peptides that have shown osteogenic or neurogenic capability as a prototypical example. X-ray photoelectron spectroscopy provides evidence that the peptide is covalently linked to the graphenic backbone. These peptide-graphene (Pep-G) conjugate materials can be processed into mechanically robust, three-dimensional constructs. Differences in their electrostatic charges allow the Pep-G conjugates to form self-assembled, layer-by-layer coatings. Further, the Pep-G conjugates are cytocompatible and electrically conductive, leading us to investigate their potential as regenerative scaffolds, as conductive surfaces can stimulate bone and nerve regeneration. Notably, PC12 cells grown on an electrically stimulated Pep-G scaffold demonstrated enhanced adhesion and neurite outgrowth compared to the control. The functionalization strategy developed here can be used to conjugate a wide variety of bioactive molecules to graphene oxide to create cell-instructive surfaces for biomedical scaffold materials.