Mitochondrial sequence reveals high levels of gene flow between breeds of domestic sheep from Asia and Europe
Mitochondrial sequence reveals high levels of gene flow between breeds of domestic sheep from Asia and Europe
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DOI：
10.1093/jhered/esi100
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发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Kijas, JW
中科院分区：
文献类型：
--
作者：
Meadows, JRS;Li, K;Kijas, JW
线粒体基因组内存在的序列变异被用来调查来自亚洲和欧洲的绵羊品种内的遗传多样性。对121只动物的2027个碱基对序列进行比较，发现了44个系统发育信息核苷酸位置和一个插入/缺失。共观察到57个单倍型，形成两个不同的分支。A型单倍型发现于来自亚洲（印度，印度尼西亚，蒙古和西藏）的品种，而B型单倍型在来自欧洲的品种（9个品种来自奥地利，埃斯特朗地，芬兰，西班牙和俄罗斯西北部）中的频率最高。单倍型的分布表明，绵羊似乎有最弱的人口结构和最高的洲际传播率的任何家畜报告的日期。只有2.7%的序列变异分布在大陆之间，低于山羊（约10%）和牛（约50%）。诊断性限制性片段长度多态性聚合酶链反应（RFLP-PCR）测试，区分A型和B单倍型被用来测试额外的223只动物从17个品种的欧洲和亚洲的起源。两个血统的混合物中发现，在每个品种，除了萨福克郡和印度加罗尔，这表明基因渗入发挥了重要作用，在品种开发和随后的选择。
Sequence variation present within the mitochondrial genome was used to investigate genetic diversity within sheep breeds from Asia and Europe. Comparison of 2027 bp of sequence from 121 animals revealed 44 phylogenetically informative nucleotide positions and a single insertion/deletion. A total of 57 haplotypes were observed which formed two distinct clades. Type A haplotypes were found in breeds from Asia (India, Indonesia, Mongolia, and Tibet), while type B haplotypes were observed at the highest frequency in breeds sourced from Europe (nine breeds from Austria, angstrom land, Finland, Spain, and northwestern Russia). The distribution of haplotypes indicates sheep appear to have the weakest population structure and the highest rate of intercontinental dispersal of any domestic animal reported to date. Only 2.7% of the sequence variation observed was partitioned between continents, which is lower than both goat (approximately 10%) and cattle (approximately 50%). Diagnostic restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) tests which distinguish type A and B haplotypes were used to test an additional 223 animals from 17 breeds of European and Asian origin. A mixture of the two lineages was found in every breed except Suffolk and the Indian Garole, indicating introgression has played a major part during breed development and subsequent selection.