Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase.
Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase.
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DOI：
10.3389/fmicb.2017.01010
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发表时间：
2017
影响因子：
5.2
通讯作者：
Yamada T
中科院分区：
文献类型：
--
作者：
Matsui T;Yoshikawa G;Mihara T;Chatchawankanphanich O;Kawasaki T;Nakano M;Fujie M;Ogata H;Yamada T
青枯雷尔氏菌（Ralstonia solanacearum）Φ RP 12和Φ RP 31是在泰国分离的巨型菌。在这里，我们表明，他们表现出相似的病毒形态，基因组组织和宿主范围。基因组比较以及系统发育树和蛋白质组学分析支持它们属于Φ KZ相关的类群，它们最近的亲缘关系是R.青枯菌（Solanacearum）的菌株为Φ RSL 2和Φ RSF 1。与Φ RSL 2和Φ RSF 1相比，Φ RP 12和Φ RP 31具有更大的基因组（约1000 bp）。280 kbp，大25%）。Φ RP 12和Φ RP 31的复制不受利福平处理（20 μg/ml）的影响，这表明噬菌体编码的RNAP在不需要宿主编码的RNAP的情况下起开始和完成这些细菌的感染周期的作用。相比之下，编码相同组RNAP的Φ RSL 2和Φ RSF 1在利福平（5 μg/ml）存在下不产生子代RNA。这一观察开启了以下可能性：在Φ RSL 2和Φ RSF 1中不存在的一些Φ RP 12/Φ RP 31因子参与了它们的宿主非依赖性转录。
Ralstonia solanacearum phages ΦRP12 and ΦRP31 are jumbo phages isolated in Thailand. Here we show that they exhibit similar virion morphology, genome organization and host range. Genome comparisons as well as phylogenetic and proteomic tree analyses support that they belong to the group of ΦKZ-related phages, with their closest relatives being R. solanacearum phages ΦRSL2 and ΦRSF1. Compared with ΦRSL2 and ΦRSF1, ΦRP12 and ΦRP31 possess larger genomes (ca. 280 kbp, 25% larger). The replication of ΦRP12 and ΦRP31 was not affected by rifampicin treatment (20 μg/ml), suggesting that phage-encoded RNAPs function to start and complete the infection cycle of these phages without the need of host-encoded RNAPs. In contrast, ΦRSL2 and ΦRSF1, encoding the same set of RNAPs, did not produce progeny phages in the presence of rifampicin (5 μg/ml). This observation opens the possibility that some ΦRP12/ΦRP31 factors that are absent in ΦRSL2 and ΦRSF1 are involved in their host-independent transcription.