Association of Cytokines With Exosomes in the Plasma of HIV-1-Seropositive Individuals
Association of Cytokines With Exosomes in the Plasma of HIV-1-Seropositive Individuals
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DOI：
10.1093/infdis/jiu676
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发表时间：
2015-06-01
影响因子：
6.4
通讯作者：
Bond, Vincent C.
中科院分区：
文献类型：
--
作者：
Konadu, Kateena Addae;Chu, Jane;Bond, Vincent C.
人类免疫缺陷病毒（HIV）感染和病毒血症个体表现出血浆细胞因子水平升高。在这里，我们表明，大多数细胞因子不是以游离形式，但似乎与外来体是从病毒粒子不同。分析纯化的外泌体以确定21种细胞因子和趋化因子的水平，并与外泌体耗尽的血浆进行比较。相对于阴性对照和血浆，大多数细胞因子在来自HIV阳性个体的外来体中显著富集。此外，将幼稚外周血单核细胞暴露于从HIV阳性患者纯化的外泌体诱导幼稚和中枢记忆CD4（+）和CD8（+）T细胞上的CD38表达，可能通过旁观者细胞活化促进炎症和病毒传播。
Human immunodeficiency virus (HIV)-infected and viremic individuals exhibit elevated levels of plasma cytokines. Here we show that most cytokines are not in free form but appear associated with exosomes that are distinct from virions. Purified exosomes were analyzed to determine the levels of 21 cytokines and chemokines and compared with exosome-depleted plasma. Most cytokines were markedly enriched in exosomes from HIV-positive individuals relative to negative controls and to plasma. Moreover, exposure of naive peripheral blood mononuclear cells to exosomes purified from HIV-positive patients induced CD38 expression on naive and central memory CD4(+) and CD8(+) T cells, probably contributing to inflammation and viral propagation via bystander cell activation.