The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus
The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus
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DOI：
10.1073/pnas.1332093100
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发表时间：
2003-06-24
影响因子：
11.1
通讯作者：
Fox, JG
中科院分区：
文献类型：
--
作者：
Suerbaum, S;Josenhans, C;Fox, JG
肝螺杆菌可引起小鼠慢性肝炎和肝癌。它是肠肝螺杆菌的原型物种，也是公认的致癌物质幽门螺杆菌的近亲。在此，我们报道了肝片吸虫ATCC51449的全基因组序列。肝片吸虫的环状染色体长1,799,146个碱基对，预测编码1,875个蛋白质。在幽门螺杆菌、空肠弯曲菌和这两种病原体中，分别有938、953和821个蛋白具有同源基因。肝毛杆菌缺乏大多数已知的幽门螺杆菌毒力因子的同源基因，包括粘附素、VacA细胞毒素和几乎所有CAG致病岛蛋白，但具有空肠弯曲菌粘附素PEB1和细胞致死性膨胀毒素(CDT)的同源基因。基因组包含一个71kb的基因组岛(HHGI1)和几个G+C含量与基因组其他部分不同的基因组岛。HHGI1编码IV型分泌系统的三个基本成分和其他毒力蛋白同源物，提示HHGI1在致病中发挥作用。利用基因芯片技术对12株肝片吸虫分离株的基因组与测序后的基因组进行比较，分析了肝片吸虫基因组的变异性。尽管包括所有已知的导致肝病的菌株在内的五种菌株与ATCC51449无法区分，但其他菌株缺乏85到229个基因，包括HHGI1的大部分，显示出物种内基因组内容的广泛差异。
Helicobacter hepaticus causes chronic hepatitis and liver cancer in mice. It is the prototype enterohepatic Helicobacter species and a close relative of Helicobacter pylori, also a recognized carcinogen. Here we report the complete genome sequence of H. hepaticus ATCC51449. H. hepaticus has a circular chromosome of 1,799,146 base pairs, predicted to encode 1,875 proteins. A total of 938, 953, and 821 proteins have orthologs in H. pylori, Campylobacter jejuni, and both pathogens, respectively. H. hepaticus lacks orthologs of most known H. pylori virulence factors, including adhesins, the VacA cytotoxin, and almost all cag pathogenicity island proteins, but has orthologs of the C. jejuni adhesin PEB1 and the cytolethal distending toxin (CDT). The genome contains a 71-kb genomic island (HHGI1) and several genomic islets whose G+C content differs from the rest of the genome. HHGI1 encodes three basic components of a type IV secretion system and other virulence protein homologs, suggesting a role of HHGI1 in pathogenicity. The genomic variability of H. hepaticus was assessed by comparing the genomes of 12 H. hepaticus strains with the sequenced genome by microarray hybridization. Although five strains, including all those known to have caused liver disease, were indistinguishable from ATCC51449, other strains lacked between 85 and 229 genes, including large parts of HHGI1, demonstrating extensive variation of genome content within the species.