Volunteer monitoring of E. coli in streams of the upper Midwestern United States: a comparison of methods
Volunteer monitoring of E. coli in streams of the upper Midwestern United States: a comparison of methods
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美国中西部上游溪流中大肠杆菌的志愿者监测：方法比较
DOI：
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发表时间：
2011
影响因子：
3
通讯作者：
Timothy S. Grant
中科院分区：
文献类型：
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作者：
Kristine F. Stepenuck;L. Wolfson;Barbara W. Liukkonen;J. Iles;Timothy S. Grant
对于饮用水或娱乐用水的人来说，粪便污染水是一个公共卫生问题。美国环保署建议使用大肠杆菌来评估娱乐淡水的粪便污染。由于可用资源有限，各州最近侧重于培训志愿人员，以扩大数据收集和资源评估。有几种细菌检测方法可供公众使用;然而，很少有研究全面评估志愿者的使用情况。本研究评价了两种E.志愿者使用的大肠杆菌监测方法：Coliscan Easygel®和3 MTM PetrifilmTM，孵育24和48小时。对这些方法进行了评估，以确定每种方法与EPA批准的实验室分析结果的匹配程度。协方差分析结果表明，当志愿者使用3 MTM PetrifilmTM监测地表水时，其结果与实验室分析结果比Coliscan Easygel®更相似。两种测试方法在预测样品是否超过或低于娱乐用表面沃茨的235 cfu/100 mL EPA人体接触标准方面具有相似的总体准确度。三分之二的志愿者更喜欢3 MTM PetrifilmTM。
Fecal contamination of water is a public health concern for those using the water for drinking or recreation. The EPA recommends using Escherichia coli to evaluate recreational freshwaters for fecal contamination. With limited resources available, states have recently focused on training volunteers to expand data collection and resource assessment. Several bacteria testing methods are available for use by the public; however, few studies have comprehensively evaluated their use by volunteers. This study evaluated two E. coli monitoring methods used by volunteers: Coliscan Easygel® and 3MTM PetrifilmTM, incubated for 24 and 48 hours. The methods were assessed to determine how closely each matched results with EPA-approved laboratory analyses. Analysis of covariance results indicated that when used by volunteers to monitor surface water, 3MTM PetrifilmTM results were more similar to laboratory analyses than Coliscan Easygel®. Both test methods had similar overall accuracy of predicting if a sample exceeded or fell below the 235 cfu/100 mL EPA body contact standard for recreational surface waters. Two-thirds of volunteers preferred 3MTM PetrifilmTM.
使用培养膜板计数水性大肠杆菌：与标准方法的比较。
影响因子：
2.4
作者：
Vail,JH;Morgan,R;Merino,CR;Gonzales,F;Miller,R;Ram,JL
通讯作者：
Ram,JL