Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
复制标题
影响真核二聚体 tRNA 前体内含子切除的突变。
DOI：
10.1002/j.1460-2075.1984.tb02013.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
D. Söll
中科院分区：
文献类型：
--
作者：
I. Willis;H. Hottinger;D. Pearson;V. Chisholm;U. Leupold;D. Söll
已经确定了一种Schizosaccharomyces pombe opal抑制丝氨酸tRNA基因（sup9‐e）和12种体内产生的突变基因的核苷酸序列，这些突变基因已经失去了抑制UGA突变的能力。对这些基因在酿酒酵母体内和体外系统中的表达分析揭示了tRNA基因转录和前体tRNA加工的缺陷。D环、内含子和额外臂的单碱基变化会影响tRNA前体剪接的效率，而反密码子干突变可能会影响这一过程的准确性。在sup9 - e基因的中间序列中发生的两个突变允许tRNA碱基配对配置的交替。sup9 - e基因和邻近的tRNAMet基因（位于下游7bp处）的转录在体内基本上被tRNASer基因的G - A19突变所消除，这表明tRNAMet可能仅通过tRNASer - tRNAMet二聚体前体的加工而产生。
The nucleotide sequences of a Schizosaccharomyces pombe opal suppressor serine tRNA gene (sup9‐e) and of 12 in vivo‐generated mutant genes, which have lost the ability to suppress UGA mutations, have been determined. Analysis of the expression of these genes in Saccharomyces cerevisiae in vitro and in vivo systems has revealed defects in tRNA gene transcription and precursor tRNA processing. Single base changes in the D‐loop, the intron and the extra arm affect the efficiency of splicing of the tRNA precursors while an anti‐codon stem mutation may affect the accuracy of this process. Two mutations which occur in the intervening sequence of the sup9‐e gene allow an alternate tRNA base pairing configuration. Transcription of the sup9‐e gene and of the adjacent tRNAMet gene (located 7 bp downstream) is essentially abolished in vivo by a G–‐A19 mutation in the tRNASer gene, suggesting that tRNAMet may be derived solely via processing of the tRNASer‐tRNAMet dimeric precursor.