INHIBITION OF PLATELET ACTIVATION AND ENDOTHELIAL-CELL INJURY BY FLAVAN-3-OL AND SAIKOSAPONIN COMPOUNDS
INHIBITION OF PLATELET ACTIVATION AND ENDOTHELIAL-CELL INJURY BY FLAVAN-3-OL AND SAIKOSAPONIN COMPOUNDS
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DOI：
10.1016/0952-3278(91)90144-t
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发表时间：
1991-09-01
影响因子：
3
通讯作者：
HSU, FL
中科院分区：
文献类型：
--
作者：
CHANG, WC;HSU, FL
本文研究了黄烷-3-醇和柴胡皂苷复合物对血小板聚集、血小板血栓素合成和H_2O_2诱导的内皮细胞损伤的影响。从茶树中分离得到7个黄烷-3-醇类化合物。中华水杉Kuntze（山茶科）和从柴胡（Bupleurum falcatum L.）（Umbelliferae）。10种化合物中，只有表没食子儿茶素和柴胡皂苷a对ADP诱导的人血小板聚集有显著的抑制作用，其抑制作用与阿司匹林相当。表没食子儿茶素和柴胡皂苷a均呈剂量依赖性地抑制外源性和内源性花生四烯酸诱导的血小板血栓素生成。在预防过氧化氢诱导的培养内皮细胞损伤中，只有没食子儿茶素-3-0-没食子酸酯和表儿茶素-3-0-没食子酸酯有效。表没食子儿茶素和柴胡皂苷a对血小板活化的抑制作用以及没食子儿茶素-3-0-没食子酸酯和表儿茶素-3-0-没食子酸酯对H_2O_2诱导的内皮细胞损伤的细胞保护作用为解释黄烷-3-醇和柴胡皂苷化合物在维持血管稳态中的可能作用提供了证据。
The effects of flavan-3-ol and saikosaponin compounds on platelet aggregation, platelet thromboxane biosynthesis and H2O2-induced endothelial cell injury were studied. Seven flavan-3-ol compounds isolated from Camellia sinensis L. var sinensis O. Kuntze (Theaceae) and three saikosaponin compounds isolated from Bupleurum falcatum L. (Umbelliferae) were used. Among the 10 compounds tested, only epigallocatechin and saikosaponin a significantly inhibited human platelet aggregation induced by ADP, and the potency of inhibition was comparable with aspirin. Both of epigallocatechin and saikosaponin a dose-dependently inhibited the platelet thromboxane formation from exogenous and endogenous arachidonic acid. In the prevention of H2O2-induced endothelial cell injury in culture, only gallocatechin-3-0-gallate and epicatechin-3-0-gallate were effective. The inhibitory effect of epigallocatechin and saikosaponin a on platelet activation and the cytoprotective effect of gallocatechin-3-0-gallate and epicatechin-3-0-gallate on H2O2-induced endothelial cell injury could give evidence of explaining the possible role of flavan-3-ol and saikosaponin compounds in maintaining vascular homeostasis.