Evaluation of antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis
Evaluation of antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis
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DOI：
10.1016/j.vetpar.2011.10.001
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发表时间：
2012-04-30
影响因子：
2.6
通讯作者：
Dalir-Naghadeh, Bahram
中科院分区：
文献类型：
--
作者：
Esmaeilnejad, Bijan;Tavassoli, Mosa;Dalir-Naghadeh, Bahram
本研究旨在评估自然感染巴氏杆菌的绵羊的抗氧化状态和氧化应激。对52只自然感染B的绵羊进行了红细胞（RBC）计数、血红蛋白（Hb）浓度、红细胞压积（PCV）、疟原虫血症率、丙二醛（MDA）浓度、红细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和总抗氧化能力（TAC）的测定。绵羊以及伊朗西阿塞拜疆省相同数量的健康绵羊。Giemsa染色外周血涂片的显微镜检查显示为B。绵羊感染利用聚合酶链反应（PCR）扩增B的部分小亚基核糖体RNA（ssu rRNA）基因序列，证实了寄生虫学诊断。52只病羊中，18只（34.61%）、11只（21.15%）、16只（30.76%）、7只（13.48%）感染率为3%。与对照组相比，感染绵羊红细胞GSH-Px、SOD、TAC和CAT活性显著降低，MDA含量显著升高。血吸虫病率与MDA呈正相关，与PCV、SOD、CAT、GSH-Px、TAC呈负相关。MDA与PCV、SOD、CAT、GSH-Px、TAC呈负相关。研究表明，B. ovis在绵羊巴贝虫病红细胞氧化损伤和贫血的发生中起重要作用。(C)2011 Elsevier B. V.保留所有权利。
The present study aimed to assess antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis. Red blood cell (RBC) count, hemoglobin (Hb) concentration, packed cell volume (PCV), piroplasm parasitemia percentage, malondialdehyde (MDA) concentration, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and total antioxidant capacity (TAC) were determined in 52 sheep naturally infected with B. ovis as well as same number of healthy sheep in West-Azerbaijan province, Iran. Microscopic examination of Giemsa-stained peripheral blood smears revealed B. ovis infection. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) analysis by amplifying a partial small subunit ribosomal RNA (ssu rRNA) gene sequence of B. ovis of 52 diseased sheep, 18 (34.61%), 11(21.15%), 16 (30.76%) and 7(13.48%) had 3% parasitemia, respectively. Compared to controls, the activities of erythrocyte GSH-Px, SOD, TAC and CAT showed a significant decrease, whereas the concentration of MDA in erythrocytes of infected sheep increased significantly. Parasitemia rate was positively correlated with MDA and negatively correlated with PCV, SOD, CAT, GSH-Px and TAC. Also, MDA was negatively correlated with PCV, SOD, catalase, GSH-Px and TAC. The study demonstrated that B. ovis plays an important role in the occurrence of oxidative damage to RBCs and anemia in ovine babesiosis. (C) 2011 Elsevier B.V. All rights reserved.