Mucosal inherent activity patterns in the rat: evidence from voltage-sensitive dyes.
Mucosal inherent activity patterns in the rat: evidence from voltage-sensitive dyes.
复制标题
大鼠粘膜固有的活动模式：来自电压敏感染料的证据。
DOI：
10.1152/jn.1995.73.1.387
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发表时间：
1995
影响因子：
2.5
通讯作者：
Tzoumaka,E
中科院分区：
文献类型：
--
作者：
Youngentob,SL;Kent,PF;Sheehe,PR;Schwob,JE;Tzoumaka,E
1.在大鼠的鼻中隔和鼻甲的内侧表面上响应于气味刺激监测染料di-4-ANEPPS的荧光变化。对于每个粘膜表面，使用10 x 10光电二极管阵列在100个连续部位处对6.0 x 6.0 mm区域进行采样。增味剂是乙酸丙酯、2-丙醇、柠檬醛、L-香芹酮和乙酰乙酸乙酯，每种都以低浓度和高浓度存在。2.像以前的工作使用光学记录技术和电位敏感染料的两栖动物上皮细胞，在大鼠制备的气味刺激引起的荧光信号几乎相同的形状，时间过程中，和响应特性的电嗅觉图（EOG）。与眼电图一样，只有在有气味刺激存在的情况下才能记录到反应（也就是说，当无气味、潮湿的空气作为刺激时，没有检测到反应）;振幅取决于有气味的浓度，乙醚和Triton X-100都能消除反应。3.虽然每个采样组织的整个范围（即，鼻中隔和鼻甲骨的内侧表面）对每种气味剂的刺激作出反应，每种刺激诱导不同的活动空间模式，该活动空间模式与气味剂浓度无关，并且在动物之间是一致的。此外，记录的鼻中隔的空间活动模式是从鼻甲内侧表面记录的空间活动模式的镜像。4.正式的统计分析的最大活动或“热点”的位点表示非常显着的影响的气味的鼻中隔和内侧表面的鼻甲。5.这些研究的结果进一步支持了这一假设，即气味质量是由不同的空间活动模式编码的嗅觉上皮细胞的特点是不同的气味。
1. Fluorescence changes in the dye di-4-ANEPPS were monitored on the rat's nasal septum and medial surface of the turbinates in response to odorant stimuli. For each mucosal surface a 6.0 x 6.0-mm area was sampled at 100 contiguous sites with a 10 x 10 photodiode array. The odorants were propyl acetate, 2-propanol, citral, L-carvone and ethylacetoacetate, each presented at a low and high concentration. 2. Like previous work using optical recording techniques and potential-sensitive dyes on the amphibian epithelium, the fluorescence signals elicited by odorant stimuli in the rat preparation were nearly identical in shape, time course, and response characteristics as the electroolfactogram (EOG). As with the EOG, a response could only be recorded in the presence of odorant stimuli (that is, no response was detected when nonodorized, humidified air was presented as the stimulus); the amplitude depended on odorant concentration, and the response was abolished both by ether and Triton X-100. 3. Although the entire expanse of each sampled tissue (i.e., septum and medial surface of the turbinates) responded to stimulation with each odorant, each stimulus induced a distinct spatial pattern of activity that was independent of odorant concentration and consistent from animal to animal. Furthermore, the spatial activity patterns recorded for the septum were mirror images of those recorded from the medial surface of the turbinates. 4. Formal statistical analysis of the loci of maximal activity or "hot spot" indicated highly significant effects of the odorants for both the septum and medial surface of the turbinates. 5. The results of these studies give further support to the hypothesis that odorant quality is encoded by differential spatial activity patterns in the olfactory epithelium that are characteristic of different odorants.