The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels
The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels
复制标题
组织工程血管中神经生长因子促进内皮祖细胞的募集
DOI：
10.1016/j.biomaterials.2009.11.037
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发表时间：
2010-03-01
期刊：
影响因子：
14
通讯作者：
Zhu, Chuhong
中科院分区：
文献类型：
--
作者：
Zeng, Wen;Yuan, Wei;Zhu, Chuhong
内皮祖细胞（EPCs）的动员和归巢是构建抗血栓和抗狭窄组织工程血管的关键。血管的生长和激活是由神经支持的。我们研究了神经生长因子（NGF）是否能促进内皮祖细胞的动员和组织工程血管的内皮化。在体外，NGF促进EPCs形成更多的集落，刺激人EPCs分化为内皮细胞，并显着增强EPCs的迁移。流式细胞仪分析显示，神经生长因子治疗增加了C57 BL/6小鼠外周循环中的EPCs数量。此外，用神经生长因子处理人内皮祖细胞促进它们在注射到小鼠体内后归巢到金属丝损伤的颈动脉中。将脱细胞大鼠血管基质与EDC交联胶原一起孵育，并使用双功能偶联剂N-琥珀酰亚胺基3-（2-吡啶基）丙酸酯（SPDP）与NGF蛋白结合。将神经生长因子结合的组织工程血管植入大鼠颈动脉1周和1个月。与对照组相比，神经生长因子结合的血管内皮化和通畅性水平显著升高。这些结果表明，NGF可以显着增加EPCs动员和归巢到血管移植物。神经营养因子如神经生长因子在体内构建组织工程血管具有治疗潜力。(C)2009爱思唯尔有限公司版权所有。
Endothelial progenitor cells (EPCs) mobilization and homing are critical to the development of an antithrombosis and anti-stenosis tissue-engineered blood vessel. The growth and activation of blood vessels are supported by nerves. We investigated whether nerve growth factors (NGF) can promote EPCs mobilization and endothelialization of tissue-engineered blood vessels. In vitro, NGF promoted EPCs to form more colonies, stimulated human EPCs to differentiate into endothelial cells, and significantly enhanced EPCs migration. Flow cytometric analysis revealed that NGF treatment increased the number of EPCs in the peripheral circulation of C57BL/6 mice. Furthermore, the treatment of human EPCs with NGF facilitated their homing into wire-injured carotid arteries after injection into mice. Decellularized rat blood vessel matrix was incubated with EDC cross-linked collagen and bound to NGF protein using the bifunctional coupling agent N-succinmidyl3-(2-pyridyidit-hio) propionate (SPDP). The NGF-bound tissue-engineered blood vessel was implanted into rat carotid artery for 1 week and 1 month. NGF-bound blood vessels possessed significantly higher levels of endothelialization and patency than controls did. These results demonstrated that NGF can markedly increase EPCs mobilization and homing to vascular grafts. Neurotrophic factors such as NGF have a therapeutic potential for the construction of tissue-engineered blood vessels in vivo. (C) 2009 Elsevier Ltd. All rights reserved.